Virtual Cloning Tutorial - Viewing 3D Structure of GFP

Open the GFP 3D Structure. This has been set up so you can see the "barrel" structure (green) and in the middle of the barrel are a few amino acids (orange) that are involved in the colour forming reaction.

Researchers have been able to alter the fluorescent colour from green to red, blue and yellow. This enables multiple proteins to be tagged at the same time and is useful for investigating interactions. The GFP "barrel" is a relative small protein and it tags easily onto either the beginning or end of the protein with as little disturbance as possible. To use a (poor) analogy, it is akin to hitching a bright green trailer (the GFP) onto a car (the protein of interest) - the trailer will follow where the car takes it.


Exercise 2: Cloning Vector
Exercise 3: Human Proinsulin
Exercise 4: Cloning Primer Design
Exercise 5: Introduce Cut Site using PCR
Exercise 6: Insert into Vector