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Virtual Cloning Tutorial: making a GFP fusion protein

This tutorial was developed by:
Dr Mike Bunce (Murdoch University, Perth, Western Australia) and the Biomatters team

In 2008 Osamu Shimomura, Martin Chalfie and Roger Y. Tsien, won a Nobel Prize in Chemistry for the discovery of Green Fluorescent Protein, GFP. Using recombinant DNA technology (cloning) allows researchers to "attach" genes to this fluorescent marker and enables visualisation of where proteins localise within a cell. In this tutorial wel will be cloning the human proinsulin gene in front of GFP gene to make what is referred to as a fusion protein.

The Nobel prize website describes the discovery of GFP and some of its uses.

If you want to open the tutorial in a different window, you can click the new window button .


Introduction: Introduction
Exercise 1: Viewing 3D structure of GFP
Exercise 2: Cloning Vector
Exercise 3: Human Proinsulin
Exercise 4: Cloning Primer Design
Exercise 5: Introduce Cut Site using PCR
Exercise 6: Insert into Vector