Virtual Cloning Tutorial - Insert into Vector

Now select the PCR product and Vector at the same time and go to Cloning→Insert into Vector


Take a look at the options which show a preview of your digested fragment and of the final ligation points.

Click OK and examine the new vector with insert included. Zoom in on the ligation point between the two CDS.

To check the translation of the fusion protein, click on the Proinsulin CDS, then hold the Shift key and click on the GFP CDS to select the region spanning both.


Click Translate to extract a separate protein sequence with the translation and check it for stop codons (*) in the sequence.

You have now completed the Virtual Cloning Tutorial.