Minimap2

A versatile mapper / pairwise aligner for genomic and spliced nucleotide sequences

Download plugin

Requires Geneious 2020.1+

    Minimap2 is a versatile aligner suited to mapping Oxford Nanopore and PacBio reads to a reference sequence. Its uses include aligning long-read sequences, aligning sequences with error rates up to ~15%, performing splice-aware alignments, and performing alignment between two closely related species with divergence below ~15%.

    Quick Start

    Install the plugin by downloading the gplugin file and dragging it in to Geneious Prime or use the plugin manager in Geneious (under Tools - Plugins in the menu).

    Once installed, run the plugin by selecting your reads and reference sequence then clicking on Align/Assemble - Map to Reference in the toolbar. Minimap2 is available as an option in the Algorithm drop-down menu.

    History

    2.1.0 (02 May 2022) – Download for Geneious 2020.1

    – Updates minimap2 to version 2.24

    2.0.0 (11 Feb 2020) – Download for Geneious 2020.1

    – Added option for specifying whether to produce secondary alignments and options for which conditions to do this in.

    1.0.0 (22 Oct 2019) – Download for Geneious 2020

    Citing

    Li, H (2018) Minimap2: pairwise alignment for nucleotide sequences. Bioinformatics, 34(18), 3094–3100