Primer Design in Geneious Pro™
Primer design is now almost an everyday task. Take the hassle of inconsistent results out of the equation. Geneious Pro™ integrates the industry leading Primer3 primer design program and extends the primer operations available to a whole new level.
Primers and Probes
- Import existing primers – Geneious Pro can import your primers in Fasta, flat file, or Genbank format. Once loaded into Geneious, choose "convert to oligo" you'll have them all as primers!
- Forward and reverse primers – Use Primer3 inside Geneious Pro to design primers to amplify any selected target region on your sequence or to amplify DNA within a specified target region
- Probe design – Design hybridization probes to any target region or entire sequence
- Design primers on alignments and assemblies – Design primers or degenerate primers on alignments and calculate the PCR product size for each sequence on the alignment independently
- Sequencing primers – Design sequencing primers across a target region of DNA that covers the entire region for a single set of parameters (coming soon)
Advanced Primer Tools
- Primer browser – Browse your primer database to easily navigate and find your primers and primer sets
- Primer database – All 'oligo' datatypes are automatically saved to the primer database allowing you manage your primers easily and to use the function test with saved primers
- Test with saved primers – Search for individual primers or primer pairs from the existing primers in your primer database that will amplify your new sequences, so you don't have to order new ones.
- Extract PCR product – Select all PCR products on a sequence or any two primers in a primer pair to extract the product of those primers.
- Trim by primers – Remove primer regions on either side of your cloning fragment
- 5' primer extension – Extend a primer with a restriction site, polyA tail or any other custom sequence
- DNA melting point – Dynamically calculate the DNA melting point for a selected region for manual primer design
