Automatically annotate plasmid maps and expression vectors
Geneious Prime is connected to PlasMapper which allows you to automatically highlight plasmids with common promoters, terminators, cloning sites, restriction sites, reporter genes, affinity tags, selectable marker genes, replication origins and open reading frames.
Codon Optimization Tool
Find rare codons or produce a fully optimized sequence based on a target organism and remove unwanted restriction sites at the same time.
Simulate molecular cloning operations in one-step
In a few clicks you can:
- Ligate multiple fragments together that have matching overhangs
- Insert a fragment of DNA into a vector with matching overhangs
- Simulate restriction cloning
- Simulate conventional PCR
- Simulate InFusion Cloning and Gibson Assembly
- Simulate TOPO cloning
- Simulate Gateway and TOPO cloning
Store and access important restriction enzyme and DNA fragment data
Drag and drop your important data, customize your restriction enzymes lists and display all of the enzymes on your linear or circular DNA fragment-all in real time. Commercially available enzymes including GoldenGate and BioBrick are already stored in Geneious Prime.
Automatically track and store important documentation
Track Cloning operations so changes can be propagated automatically from parents to all descendants.
Gibson Assembly – Learn how to simulate Gibson Assembly in Geneious Prime.
TOPO Cloning – Learn how to simulate TOPO cloning in Geneious Prime, using TA, Blunt or Directional methods.
Restriction Cloning – This tutorial shows you how to use the restriction cloning tools in Geneious Prime to create a plasmid with a functional GFP fusion protein.
Golden Gate Cloning – Learn how to simulate Golden Gate cloning, including how to automatically design oligonucleotide primers for generating the overhangs for assembly of parts.
Homology-Based Cloning Methods – Design experiments using exonuclease-based recombination (3’ or 5’ exonuclease) or using homologous recombination.
More Geneious Prime Features
Primer Design – Design primers, find CRISPR sites, and optimize codons.
Assembly and Mapping – De novo assembly or reference mapping using industry leading algorithms, including TopHat and Velvet.
NGS Visualization and Analysis – Comprehensive analysis of data, including genome browser, contig visualization, SNP calling, and RNA-Seq expression analysis.
Alignment – Perform pairwise and multiple alignments of DNA or protein using trusted algorithms, including MAFFT and ClustalW.
BLAST / NCBI – Connect to NCBI and PubMed, submit sequences directly to GenBank, BLAST sequences and search your own database.
Import Data – Import and convert common file types as well as their annotations and notes with a simple drag and drop
I have found Geneious to be one of the most intuitive and powerful sequence analysis programs that I have used in all my years working in the molecular biology field. Thanks for making my life so much easier.
Geneious is an absolutely beautiful package to work with. We love it and we are telling everyone about it.
This program is wonderful! I was desperately looking for a software program that would make analyzing my sequencing data less painful. Thank you for developing a quality, user-friendly program. The interface is great!
Geneious is paramount in my research so I can’t be without it for too long.
The combination of powerful analytic features, intuitive navigation and file organization, and the responsiveness that representatives have shown to the inclusion of new functions has made us big fans of this software.
Geneious is a great software… I just want to express my appreciation to Biomatters to come up with such good product.
For many years I’ve wanted a friendly, menu-driven interface to our Glimmer program, but my research group has never had the resources available to build one. The Geneious Glimmer interface is terrific; it will allow any scientist to find genes in almost any bacterial, viral, or archaeal sequence, without requiring background expertise in Unix command-line programs. I’m also very pleased that Geneious makes Glimmer available across multiple computer platforms, including Windows and Mac.
We’ve been using our Geneious licenses a lot and really like the software. We’ve written several plug-ins ourselves and are using it more and more to analyze our data.
It’s not just about the buttons. From a user’s perspective, Phobos generates annotations, and Geneious handles annotations beautifully. The integration was obvious! And from a technical perspective it was easy to achieve, because it was simple to parse the outputs from Phobos into repeat annotations for the Geneious interface.
TopHat is a robust tool, but it was designed with experienced bioinformatics scientists in mind. Geneious makes TopHat accessible to many more users through the same powerful, intuitive user interface they have with other Geneious analysis tasks.