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Getting Started

Quick Start to Geneious Prime

VIDEO

Quick Start

Get up and running quickly with this brief tour of the Geneious Prime interface

Getting Started with Geneious Prime

VIDEO SERIES

Getting Started Series

Master the basics of Geneious Prime with short video tutorials of key features

Getting Started with Biologics [Thumb]

VIDEO SERIES

Get Started with Biologics

Master the basics of Geneious Biologics with this video series of key features

Sanger Sequencing and Microsatellites

How to perform Sanger Sequencing

VIDEO SERIES

Sanger Sequence Analysis

Align your chromatograms to a reference sequence, find variants and verify sequences.

Tutorial Assembling Chromatograms

WRITTEN TUTORIAL

Assembling Chromatograms

Trim, edit and assemble chromatograms, find heterozygotes and incorrectly called bases.

How to perform Microsatellite Analysis

VIDEO SERIES

Microsatellite Analysis

Learn how to fit ladders, call peaks, predict bins and display alleles in tabular format with this vidoe series.

Tutorial microsatellite analysis

WRITTEN TUTORIAL

Microsatellite Traces

Use the Microsatellite plugin to fit a ladder, call peaks, bin alleles and produce a table of genotypes.

Next Generation Sequencing

How to perform de novo assembly

VIDEO SERIES

De Novo Assembly

Learn to perform de novo assembly of short-read NGS data and assemble circular contigs.

Tutorial de novo assembly

WRITTEN TUTORIAL

Perform De Novo Assembly

De novo assembly of short-read NGS data and quality checking your assembly against a reference sequence.

How to map to reference

VIDEO SERIES

Map to Reference

Map next-generation sequencing data against a reference, and assemble coronavirus genomes.

Tutorial mapping and SNP Calling

WRITTEN TUTORIAL

Mapping and SNP Calling

Perform a reference assembly with NGS data and call SNPs on the assembled contig.

How to perform metagenomics analysis

VIDEO SERIES

Metagenomics Analysis

Learn how to assemble, filter and analyze an NGS amplicon metagenomic data set.

Tutorial metagenomic analysis

WRITTEN TUTORIAL

Amplicon Metagenomics

Assemble, filter and analyze an NGS amplicon metagenomic data set.

Tutorial sequence classifier

CASE STUDY

Sequence Classifier

Use Sequence Classifier to classify mitochondrial sequences obtained from subfossil bones.

How to perform expression analysis

VIDEO SERIES

Expression Analysis

Calculate and compare normalized expression measures from RNA-Seq data.

Tutorial expression analysis

WRITTEN TUTORIAL

Perform Expression Analysis

Calculate normalized expression levels from RNA-Seq data.

Tutorial expression analysis with DESeq2

WRITTEN TUTORIAL

Expression Analysis with DESeq2

Use DESeq2 to compare expression levels for two sample conditions with replicates.

Alignments and Tree Building

How to build phylogenetic trees

VIDEO SERIES

Phylogenetic Tree Building

Learn the basic steps to build a tree and manipulate the tree viewer in Geneious Prime.

How to perform multiple alignments

VIDEO SERIES

Multiple Alignments

Use multiple alignments to assess levels of homology among a group of sequences before constructing a phylogeny.

Tutorial building phylogenetic trees

WRITTEN TUTORIAL

Building Phylogenetic Trees

Align sequences, build a phylogenetic tree, and view and manipulate the tree.

How to perform pairwise alignment

VIDEO SERIES

Pairwise Alignments

Use a dot plot to view regions of similarity and perform pairwise alignment of related sequences.

Tutorial pairwise alignment

WRITTEN TUTORIAL

Perform Pairwise Alignments

Align pairs of DNA and protein sequences using dot plots and alignment algorithms.

How to align Genomes with Mauve

VIDEO SERIES

Mauve Alignment

Align bacterial genomes with the Mauve plugin. Convert Mauve alignments into standard alignments.

Tutorial aligning bacterial genomes with mauve

WRITTEN TUTORIAL

Perform Mauve Alignments

Use the Mauve plugin to align bacterial genomes, complete genomes, and ordering of draft genomes against a reference.

Tutorial plant molecular evolution

CASE STUDY

Plant Molecular Evolution

Annotate plant DNA sequences using BLAST and multiple alignment, then read a phylogenetic tree.

Annotate and Visualize

How to annotate sequences

VIDEO SERIES

Annotate Sequences

Annotate your sequences by customizing the view, adding new annotations or annotating your sequence using BLAST.

Tutorial transferring annotations

WRITTEN TUTORIAL

Transfer Annotations

Quickly annotate a sequence by transferring annotations from related sequences with these three easy methods.

How to visualize 3D structures

VIDEO SERIES

Visualize 3D Structures

Learn to use the Geneious 3D structure viewer to visualize and manipulate active sites in a protein structure.

Tutorial visualizing 3D structures

WRITTEN TUTORIAL

3D Structures

Explore the controls in the Geneious 3D structure viewer and visualize active sites in a protein structure.

Molecular Cloning

How to perform restriction cloning

VIDEO SERIES

Restriction Cloning

Find and analyze restriction enzymes and simulate restriction cloning of a single insert into a vector backbone.

Tutorial restriction cloning

WRITTEN TUTORIAL

Perform Restriction Cloning

Simulate restriction cloning, ligating PCR product into an expression vector and confirm CDS in-frame fusion.

How to perform gateway cloning

VIDEO SERIES

Gateway Cloning

Learn how Gateway cloning works and how to clone single or multiple fragments into an expression clone.

Tutorial gateway cloning

WRITTEN TUTORIAL

Perform Gateway Cloning

Simulate Gateway cloning, automatically design attB-linked primers, BP and LR reactions, and Multisite Gateway.

How to perform golden gate cloning

VIDEO SERIES

Golden Gate Cloning

Learn the principles of Golden Gate cloning and how to simulate standard or batch Golden Gate cloning.

Tutorial golden gate cloning

WRITTEN TUTORIAL

Perform Golden Gate Cloning

Simulate Golden Gate cloning and automatically design primers for generating overhangs for assembly of parts.

How to perform gibson assembly

VIDEO SERIES

Gibson Assembly

Simulate Gibson Assembly of a single insert into a vector, and perform batch cloning from a sequence list.

Tutorial gibson assembly

WRITTEN TUTORIAL

Perform Gibson Assembly

Simulate Gibson Assembly with the assembly of single and multiple fragments.

How to perform in-fusion cloning

VIDEO SERIES

In-Fusion Cloning

Learn how In-Fusion cloning works, how to perform In-Fusion cloning in Geneious Prime and analyze your results.

How to perform TOPO cloning

VIDEO SERIES

TOPO Cloning

How to simulate TOPO cloning using TA, Blunt or Directional methods.

Tutorial TOPO cloning

WRITTEN TUTORIAL

Perform TOPO Cloning

Simulate TOPO cloning using TA, Blunt or Directional methods.

How to perform parts cloning

VIDEO SERIES

Parts Cloning

Simulate parts cloning and concatenate libraries of genetic elements outputting all possible combinations.

Primer Design and CRISPR

How to perform primer design

VIDEO SERIES

Primer Design

Learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.

Tutorial primer design

WRITTEN TUTORIAL

Perform Primer Design

Import, design and test oligonucleotide primers, primer-pairs and primer-pair/probe combinations.

How to work with CRISPR

VIDEO SERIES

Working with CRISPR

How to find and annotate CRISPR sites, then analyze and interpret the results.

Tutorial finding CRISPR sites

WRITTEN TUTORIAL

Finding CRISPR Sites

Find and annotate CRISPR sites in a sequence and check against a genome for off-target binding sites.

Tutorial Analyze CRISPR editing results

WRITTEN TUTORIAL

Analyze CRISPR Editing

Analyze CRISPR editing results, including how to prepare your sequence files, set up the analysis and interpret the results.

BLAST, NCBI & GenBank

How to perform BLAST searching

VIDEO SERIES

BLAST Searching

How to single or batch BLAST your sequence against GenBank or custom databases to find similar sequences.

Tutorial BLAST searching

WRITTEN TUTORIAL

Perform BLAST Searching

BLAST your sequence against GenBank or custom databases to find similar sequences.

How to work with GenBank

VIDEO SERIES

Working with GenBank

Access GenBank, and prepare and submit sequences and alignments to the GenBank database.

Tutorial accessing GenBank

WRITTEN TUTORIAL

Accessing GenBank

Access information in GenBank, including sequences, taxonomic information and publications.

Tutorial GenBank Submission

WRITTEN TUTORIAL

GenBank Submission

Format sequences and alignments for submission to GenBank using the Geneious GenBank Submission tool.

Automation & Customization

How to create automated workflows

VIDEO SERIES

Automated Workflows

Use workflows to automate your analyses and run a set of operations with a single click.

Tutorial creating automated workflows

WRITTEN TUTORIAL

Creating Workflows

Set up a workflow to automatically run a pipeline from raw Sanger sequences to a phylogenetic tree.

Using command line interface

DOCUMENTATION

Command Line Interface

Sync data and run Geneious Prime operations and workflows locally or execute on more powerful servers.

Plugin development

DOCUMENTATION

Plugin Development

Use the Geneious Public API to create plugins to extend functionality, integrate with existing systems or add your own custom algorithms