Master Geneious and key concepts in molecular biology and bioinformatics.
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Getting Started
VIDEO
Tour Geneious Prime
Get up and running quickly with this brief tour of the Geneious Prime interface
VIDEO SERIES
Getting Started Series
Master the basics of Geneious Prime with short video tutorials of key features
VIDEO SERIES
Get Started with Geneious Biologics
Master the basics of Geneious Biologics with this video series
Sanger Sequencing and Microsatellites
VIDEO SERIES
Sanger Sequence Analysis
Align your chromatograms to a reference sequence, find variants and verify sequences.
WRITTEN TUTORIAL
Assembling Chromatograms
Trim, edit and assemble chromatograms, find heterozygotes and incorrectly called bases.
VIDEO SERIES
Microsatellite Analysis
Learn how to fit ladders, call peaks, predict bins and display alleles in tabular format with this vidoe series.
WRITTEN TUTORIAL
Microsatellite Traces
Use the Microsatellite plugin to fit a ladder, call peaks, bin alleles and produce a table of genotypes.
Next Generation Sequencing
VIDEO SERIES
De Novo Assembly
Learn to perform de novo assembly of short-read NGS data and assemble circular contigs.
WRITTEN TUTORIAL
Perform De Novo Assembly
De novo assembly of short-read NGS data and quality checking your assembly against a reference sequence.
VIDEO SERIES
Map to Reference
Map next-generation sequencing data against a reference, and assemble coronavirus genomes.
VIDEO SERIES
Compare and Call SNPs
Perform a reference assembly with next-generation sequencing (NGS) data and call SNPs on the assembled contig.
WRITTEN TUTORIAL
Mapping and SNP Calling
Perform a reference assembly with NGS data and call SNPs on the assembled contig.
VIDEO SERIES
Metagenomics Analysis
Learn how to assemble, filter and analyze an NGS amplicon metagenomic data set.
WRITTEN TUTORIAL
Amplicon Metagenomics
Assemble, filter and analyze an NGS amplicon metagenomic data set.
CASE STUDY
Sequence Classifier
Use Sequence Classifier to classify mitochondrial sequences obtained from subfossil bones.
VIDEO SERIES
Expression Analysis
Calculate and compare normalized expression measures from RNA-Seq data
WRITTEN TUTORIAL
Perform Expression Analysis
Calculate normalized expression levels from RNA-Seq data.
WRITTEN TUTORIAL
Expression Analysis with DESeq2
Use DESeq2 to compare expression levels for two sample conditions with replicates.
Alignments and Tree Building
VIDEO SERIES
Phylogenetic Tree Building
Learn the basic steps to build a tree and manipulate the tree viewer in Geneious Prime.
VIDEO SERIES
Multiple Alignments
Use multiple alignments to assess levels of homology among a group of sequences before constructing a phylogeny.
WRITTEN TUTORIAL
Building Phylogenetic Trees
Align sequences, build a phylogenetic tree, and view and manipulate the tree.
VIDEO SERIES
Pairwise Alignments
Use a dot plot to view regions of similarity and perform pairwise alignment of related sequences.
WRITTEN TUTORIAL
Perform Pairwise Alignments
Align pairs of DNA and protein sequences using dot plots and alignment algorithms.
VIDEO SERIES
Mauve Alignment
Align bacterial genomes with the Mauve plugin. Convert Mauve alignments into standard alignments.
WRITTEN TUTORIAL
Perform Mauve Alignments
Use the Mauve plugin to align bacterial genomes, complete genomes, and ordering of draft genomes against a reference.
CASE STUDY
Plant Molecular Evolution
Annotate plant DNA sequences using BLAST and multiple alignment, then read a phylogenetic tree.
Annotate and Visualize
VIDEO SERIES
Annotate Sequences
Annotate your sequences by customizing the view, adding new annotations or annotating your sequence using BLAST.
WRITTEN TUTORIAL
Transfer Annotations
Quickly annotate a sequence by transferring annotations from related sequences with these three easy methods.
VIDEO SERIES
Visualize 3D Structures
Learn to use the Geneious 3D structure viewer to visualize and manipulate active sites in a protein structure.
WRITTEN TUTORIAL
3D Structures
Explore the controls in the Geneious 3D structure viewer and visualize active sites in a protein structure.
Molecular Cloning
VIDEO SERIES
Restriction Cloning
Find and analyze restriction enzymes and simulate restriction cloning of a single insert into a vector backbone.
WRITTEN TUTORIAL
Perform Restriction Cloning
Simulate restriction cloning, ligating PCR product into an expression vector and confirm CDS in-frame fusion.
VIDEO SERIES
Gateway Cloning
Learn how Gateway cloning works and how to clone single or multiple fragments into an expression clone.
WRITTEN TUTORIAL
Perform Gateway Cloning
Simulate Gateway cloning, automatically design attB-linked primers, BP and LR reactions, and Multisite Gateway.
VIDEO SERIES
Golden Gate Cloning
Learn the principles of Golden Gate cloning and how to simulate standard or batch Golden Gate cloning.
WRITTEN TUTORIAL
Perform Golden Gate Cloning
Simulate Golden Gate cloning and automatically design primers for generating overhangs for assembly of parts.
VIDEO SERIES
Gibson Assembly
Simulate Gibson Assembly of a single insert into a vector, and perform batch cloning from a sequence list.
WRITTEN TUTORIAL
Perform Gibson Assembly
Simulate Gibson Assembly with the assembly of single and multiple fragments.
VIDEO SERIES
In-Fusion Cloning
Learn how In-Fusion cloning works, how to perform In-Fusion cloning in Geneious Prime and analyze your results.
VIDEO SERIES
Parts Cloning
Simulate parts cloning and concatenate libraries of genetic elements outputting all possible combinations.
Primer Design and CRISPR
VIDEO SERIES
Primer Design
Learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.
WRITTEN TUTORIAL
Perform Primer Design
Import, design and test oligonucleotide primers, primer-pairs and primer-pair/probe combinations.
VIDEO SERIES
Working with CRISPR
How to find and annotate CRISPR sites, then analyze and interpret the results.
WRITTEN TUTORIAL
Finding CRISPR Sites
Find and annotate CRISPR sites in a sequence and check against a genome for off-target binding sites.
WRITTEN TUTORIAL
Analyze CRISPR Editing
Analyze CRISPR editing results, including how to prepare your sequence files, set up the analysis and interpret the results.
BLAST, NCBI & GenBank
VIDEO SERIES
BLAST Searching
How to single or batch BLAST your sequence against GenBank or custom databases to find similar sequences.
WRITTEN TUTORIAL
Perform BLAST Searching
BLAST your sequence against GenBank or custom databases to find similar sequences.
VIDEO SERIES
Working with GenBank
Access GenBank, and prepare and submit sequences and alignments to the GenBank database.
WRITTEN TUTORIAL
Accessing GenBank
Access information in GenBank, including sequences, taxonomic information and publications.
WRITTEN TUTORIAL
GenBank Submission
Format sequences and alignments for submission to GenBank using the Geneious GenBank Submission tool.
Automation & Customization
VIDEO SERIES
Automated Workflows
Use workflows to automate your analyses and run a set of operations with a single click.
WRITTEN TUTORIAL
Creating Workflows
Set up a workflow to automatically run a pipeline from raw Sanger sequences to a phylogenetic tree.
DOCUMENTATION
Command Line Interface
Sync data and run Geneious Prime operations and workflows locally or execute on more powerful servers.
DOCUMENTATION
Plugin Development
Use the Geneious Public API to create plugins to extend functionality, integrate with existing systems or add your own custom algorithms