Master Geneious and key concepts in molecular biology and bioinformatics.
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Tour Geneious Prime
Get up and running quickly with this brief tour of the Geneious Prime interface
Getting Started Series
Master the basics of Geneious Prime with short video tutorials of key features
Get Started with Geneious Biologics
Master the basics of Geneious Biologics with this video series
Sanger Sequencing and Microsatellites
Sanger Sequence Analysis
Align your chromatograms to a reference sequence, find variants and verify sequences.
Trim, edit and assemble chromatograms, find heterozygotes and incorrectly called bases.
Learn how to fit ladders, call peaks, predict bins and display alleles in tabular format with this vidoe series.
Use the Microsatellite plugin to fit a ladder, call peaks, bin alleles and produce a table of genotypes.
Next Generation Sequencing
De Novo Assembly
Learn to perform de novo assembly of short-read NGS data and assemble circular contigs.
Perform De Novo Assembly
De novo assembly of short-read NGS data and quality checking your assembly against a reference sequence.
Map to Reference
Map next-generation sequencing data against a reference, and assemble coronavirus genomes.
Compare and Call SNPs
Perform a reference assembly with next-generation sequencing (NGS) data and call SNPs on the assembled contig.
Mapping and SNP Calling
Perform a reference assembly with NGS data and call SNPs on the assembled contig.
Learn how to assemble, filter and analyze an NGS amplicon metagenomic data set.
Assemble, filter and analyze an NGS amplicon metagenomic data set.
Use Sequence Classifier to classify mitochondrial sequences obtained from subfossil bones.
Calculate and compare normalized expression measures from RNA-Seq data
Perform Expression Analysis
Calculate normalized expression levels from RNA-Seq data.
Expression Analysis with DESeq2
Use DESeq2 to compare expression levels for two sample conditions with replicates.
Alignments and Tree Building
Phylogenetic Tree Building
Learn the basic steps to build a tree and manipulate the tree viewer in Geneious Prime.
Use multiple alignments to assess levels of homology among a group of sequences before constructing a phylogeny.
Building Phylogenetic Trees
Align sequences, build a phylogenetic tree, and view and manipulate the tree.
Use a dot plot to view regions of similarity and perform pairwise alignment of related sequences.
Perform Pairwise Alignments
Align pairs of DNA and protein sequences using dot plots and alignment algorithms.
Align bacterial genomes with the Mauve plugin. Convert Mauve alignments into standard alignments.
Perform Mauve Alignments
Use the Mauve plugin to align bacterial genomes, complete genomes, and ordering of draft genomes against a reference.
Plant Molecular Evolution
Annotate plant DNA sequences using BLAST and multiple alignment, then read a phylogenetic tree.
Annotate and Visualize
Annotate your sequences by customizing the view, adding new annotations or annotating your sequence using BLAST.
Quickly annotate a sequence by transferring annotations from related sequences with these three easy methods.
Visualize 3D Structures
Learn to use the Geneious 3D structure viewer to visualize and manipulate active sites in a protein structure.
Explore the controls in the Geneious 3D structure viewer and visualize active sites in a protein structure.
Find and analyze restriction enzymes and simulate restriction cloning of a single insert into a vector backbone.
Perform Restriction Cloning
Simulate restriction cloning, ligating PCR product into an expression vector and confirm CDS in-frame fusion.
Learn how Gateway cloning works and how to clone single or multiple fragments into an expression clone.
Perform Gateway Cloning
Simulate Gateway cloning, automatically design attB-linked primers, BP and LR reactions, and Multisite Gateway.
Golden Gate Cloning
Learn the principles of Golden Gate cloning and how to simulate standard or batch Golden Gate cloning.
Perform Golden Gate Cloning
Simulate Golden Gate cloning and automatically design primers for generating overhangs for assembly of parts.
Simulate Gibson Assembly of a single insert into a vector, and perform batch cloning from a sequence list.
Perform Gibson Assembly
Simulate Gibson Assembly with the assembly of single and multiple fragments.
Learn how In-Fusion cloning works, how to perform In-Fusion cloning in Geneious Prime and analyze your results.
Simulate parts cloning and concatenate libraries of genetic elements outputting all possible combinations.
Primer Design and CRISPR
Learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.
Perform Primer Design
Import, design and test oligonucleotide primers, primer-pairs and primer-pair/probe combinations.
Working with CRISPR
How to find and annotate CRISPR sites, then analyze and interpret the results.
Finding CRISPR Sites
Find and annotate CRISPR sites in a sequence and check against a genome for off-target binding sites.
Analyze CRISPR Editing
Analyze CRISPR editing results, including how to prepare your sequence files, set up the analysis and interpret the results.
BLAST, NCBI & GenBank
How to single or batch BLAST your sequence against GenBank or custom databases to find similar sequences.
Perform BLAST Searching
BLAST your sequence against GenBank or custom databases to find similar sequences.
Working with GenBank
Access GenBank, and prepare and submit sequences and alignments to the GenBank database.
Access information in GenBank, including sequences, taxonomic information and publications.
Format sequences and alignments for submission to GenBank using the Geneious GenBank Submission tool.
Automation & Customization
Use workflows to automate your analyses and run a set of operations with a single click.
Set up a workflow to automatically run a pipeline from raw Sanger sequences to a phylogenetic tree.
Command Line Interface
Sync data and run Geneious Prime operations and workflows locally or execute on more powerful servers.
Use the Geneious Public API to create plugins to extend functionality, integrate with existing systems or add your own custom algorithms