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Working with CRISPR

How to find, annotate and check CRISPR sites, prepare sequence files, analyze, and interpret the results.

Finding CRISPR Sites

INSIDE THE VIDEO
Use the CRISPR tool in Geneious Prime to find and annotate CRISPR sites in a sequence and check for off-target binding sites.

LENGTH
5 minutes

Finding Paired CRISPR Sites

INSIDE THE VIDEO
Use the CRISPR tool in Geneious Prime to find paired sites in a sequence.

LENGTH
3 minutes

Merging Paired Reads

INSIDE THE VIDEO
Learn how to prepare your data for CRISPR analysis by merging paired reads.

LENGTH
2 minutes

Analyze CRISPR Editing Results

INSIDE THE VIDEO
Analyze CRISPR editing results in Geneious Prime, including how to prepare your sequence files, set up the analysis, and interpret the results.

LENGTH
3 minutes

Recommended Resources

CRISPR

TUTORIAL
Practice Finding CRISPR Sites

Find and annotate CRISPR sites in a sequence with these practical exercises.

TUTORIAL
Practice Analyzing CRISPR Results

Analyze CRISPR editing results with these practical exercises.

MANUAL
Instructions for CRISPR

A guide to using the CRISPR tools in Geneious Prime.

FAQs
How do I set the off-target database for CRISPR?

Learn to search for off-target binding sites against a database of sequences.

GENEIOUS ACADEMY

How to perform primer design
How to perform restriction cloning
How to annotate sequences
How to perform Sanger Sequencing