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Golden Gate Cloning

Learn the principles of Golden Gate cloning and how to simulate standard or batch Golden Gate cloning.

Performing Golden Gate Cloning

INSIDE THE VIDEO
Assess your DNA sequences, arrange your fragments and design primers, simulate Golden Gate cloning, and check your assembly.

  • Sequence assessment
  • Performing the assembly
  • Checking the assembly

LENGTH
6 minutes

Batch Golden Gate Cloning

INSIDE THE VIDEO
Perform cloning using a sequence list, rearrange parts, simulate Golden Gate cloning, and view your final product and primers.

LENGTH
2 minutes

Recommended Resources

GOLDEN GATE CLONING

TUTORIAL
Practice Golden Gate

Practical exercises for performing batch and standard Golden Gate Cloning.

FAQ
How do I create a custom enzyme set?

Learn how to create a specific set of enzymes that you want to use repeatedly.

MANUAL
Instructions for Golden Gate

A guide to using the Golden Gate cloning tool in Geneious.

VIDEO SERIES
Find and Analyze Restriction Enzymes

How to find, add, remove and analyze restriction sites and the resulting fragments.

GENEIOUS ACADEMY

How to perform gateway cloning
How to perform restriction cloning
How to perform parts cloning
How to perform primer design