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Primer Design

Learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.

How to Design Primers

INSIDE THE VIDEO
Automatically design new primers, adjust melting temperature, length, hairpin and GC content, and extract PCR product from a primer pair.

LENGTH
6 minutes

Importing Primers

INSIDE THE VIDEO
Learn to import primer sequences to Geneious Prime using a delimited text file or from a spreadsheet by copying and pasting

LENGTH
3 minutes

Test with Saved Primers

INSIDE THE VIDEO
Learn the two methods for testing primers against a target sequence: add primers to a sequence or test with saved primers.

LENGTH
3 minutes

Design Primers with 5′ extensions

INSIDE THE VIDEO
Learn three ways to add 5-prime extensions, manually, with the design new primers tool or by editing existing primer sequences.

LENGTH
4 minutes

Recommended Resources

PRIMER DESIGN

TUTORIAL
Primer Design

Import, design and test primers with this series of practical exercises.

FAQ
How to use Primers

Common questions on using primers in Geneious.

MANUAL
Instructions for Primer Tools

A guide to using the primer tools in Geneious.

FEATURES
Primer Design

PCR and sequencing primer design tool. Primer database and testing.

GENEIOUS ACADEMY

How to work with CRISPR
How to perform gateway cloning
How to perform BLAST searching
How to perform Sanger Sequencing