LEARN GENEIOUS
Assembly and Mapping
Watch videos and complete tutorials to learn how to assemble and map NGS reads and chromatogram sequences.
Intro to Sanger Sequencing
INSIDE THE VIDEO
Align your chromatograms to a reference sequence so you can find variants and verify sequences.
LEARN HOW TO:
- Prepare your data including checking sequence quality and trimming the ends
- Map a chromatogram sequence against a reference sequence
- View the contig, change your display and edit bases
De Novo Assembly of NGS Reads
INSIDE THE VIDEO
Perform a de novo assembly of short-read next-generation sequencing data
LEARN HOW TO:
- De novo assemble paired end data using the Geneious de novo assembler
- Compare full and normalized data sets
- View the assembly results and consensus sequence
Explore Map to Reference Contig Documents
INSIDE THE VIDEO
Explore the contig document after mapping NGS data to a reference in Geneious Prime
LEARN HOW TO:
- Viewing the consensus sequence
- Quickly identifying regions of high or low coverage using the coverage graph
Assembling Coronavirus Genomes
INSIDE THE VIDEO
A workflow for assembly of coronavirus genomes generated by tiled amplicon RT-PCR followed by Illumina sequencing
LEARN HOW TO:
Circular Contig Assembly
INSIDE THE VIDEO
Create circular contigs with the Geneious de novo assembler for more accurate assembly of circular genomes
LEARN HOW TO:
- Assemble mitochondria, chloroplast or bacterial genomes
- Allow reads to span across the ends of your assembly and wrap around the origin
TUTORIALS
Assembling Chromatograms
Learn how to edit and assemble chromatograms produced from a Sanger sequencing run for downstream analyses
De Novo Assembly
Learn how to perform a de novo assembly of short-read next-generation sequencing data.
Mapping and SNP Calling
Learn how to perform a reference assembly with NGS data and to call SNPs on the assembled contig.