LEARN GENEIOUS

Assembly and Mapping

Watch videos and complete tutorials to learn how to assemble and map NGS reads and chromatogram sequences.

Intro to Sanger Sequencing

INSIDE THE VIDEO
Align your chromatograms to a reference sequence so you can find variants and verify sequences.

LEARN HOW TO:

  • Prepare your data including checking sequence quality and trimming the ends
  • Map a chromatogram sequence against a reference sequence
  • View the contig, change your display and edit bases

De Novo Assembly of NGS Reads

INSIDE THE VIDEO
Perform a de novo assembly of short-read next-generation sequencing data

LEARN HOW TO:

  • De novo assemble paired end data using the Geneious de novo assembler
  • Compare full and normalized data sets
  • View the assembly results and consensus sequence

How To Map NGS Reads to a Reference

INSIDE THE VIDEO
Map next generation sequencing data against a reference sequence using the map to reference tool

LEARN HOW TO:

  • Select a reference sequence
  • Choose a mapping algorithm
  • Set the sensitivity

Explore Map to Reference Contig Documents

INSIDE THE VIDEO
Explore the contig document after mapping NGS data to a reference in Geneious Prime

LEARN HOW TO:

  • Viewing the consensus sequence
  • Quickly identifying regions of high or low coverage using the coverage graph

Assembling Coronavirus Genomes

INSIDE THE VIDEO
A workflow for assembly of coronavirus genomes generated by tiled amplicon RT-PCR followed by Illumina sequencing

LEARN HOW TO:

  • Remove poor quality bases and the PCR primers used to generate tiled amplicons
  • Map your trimmed reads to the reference SARS-CoV-2 genome
  • Call SNPs and generate a consensus sequence

Circular Contig Assembly

INSIDE THE VIDEO
Create circular contigs with the Geneious de novo assembler for more accurate assembly of circular genomes

LEARN HOW TO:

  • Assemble mitochondria, chloroplast or bacterial genomes
  • Allow reads to span across the ends of your assembly and wrap around the origin

TUTORIALS

Prime - Sanger Alignment
Assembling Chromatograms

Learn how to edit and assemble chromatograms produced from a Sanger sequencing run for downstream analyses

Start Tutorial >

Sequence_Assembly
De Novo Assembly

Learn how to perform a de novo assembly of short-read next-generation sequencing data.

Start Tutorial >

Assembly_and_Mapping
Mapping and SNP Calling

Learn how to perform a reference assembly with NGS data and to call SNPs on the assembled contig.

Start Tutorial >