LEARN GENEIOUS

Cloning, CRISPR and Primer Design

Watch videos and complete tutorials to learn how to design primers, simulate cloning and find and analyze CRISPR sites

How To Simulate Restriction Cloning

INSIDE THE VIDEO
Simulate restriction cloning of a single insert into a vector backbone.

LEARN HOW TO:

  • Select a vector and insert for restriction cloning
  • Select enzymes from annotated enzymes, an enzyme set or from the enzyme drop down menu
  • Add additional inserts and rearrange inserts
  • View the final cloning product including annotations and concatenated sequence

How To Find and Analyze Restriction Enzymes

INSIDE THE VIDEO
Find, add, remove and analyze restriction sites and the resulting fragments

LEARN HOW TO:

  • Select enzymes by enzyme set or characteristics
  • Display a table of all enzymes and information
  • Simulate an agarose gel using chosen restriction enzymes
  • View and export fragments information
  • Create your own custom enzyme set

How To Annotate Sequences

INSIDE THE VIDEO
Customize the viewing of your annotations and create new annotations from existing files or databases.

LEARN HOW TO:

  • Display annotations on your sequence or in a table
  • Import annotation files
  • Manually add annotations
  • Automatically add annotations from the reference feature database
  • Transfer annotations from a similar sequence

TUTORIALS

Cloning - Primer Tutorial
Primer Design

Learn how to import, design and test oligonucleotide primers, primer-pairs and primer-pair/probe combinations

Start Tutorial >

Cloning - Find CRISPR Sites
Finding CRISPR Sites

Learn how to find and annotate CRISPR sites in a sequence and check against a genome for off-target binding sites.

Start Tutorial >

Cloning - Analyze CRISPR Editing Results
Analyze CRISPR Editing

Learn how to analyze CRISPR editing results, including how to prepare your sequence files, set up the analysis and interpret the results.

Start Tutorial >

Cloning - Restriction Cloning
Restriction Cloning

Learn how to simulate restriction cloning including ligating a PCR product into an expression vector and confirming the CDS in-frame fusion.

Start Tutorial >

Cloning - Golden Gate
Golden Gate Cloning

Learn how to simulate Golden Gate cloning, including how to automatically design primers for generating overhangs.

Start Tutorial >

Gateway Cloning

Learn how to simulate Gateway cloning, including designing attB-linked oligonucleotide primers, BP and LR reactions, and Multisite Gateway cloning.

Start Tutorial >

Gibson Assembly

Learn how to simulate Gibson Assembly in Geneious Prime.

Start Tutorial >

TOPO Cloning

Learn how to simulate TOPO cloning in Geneious Prime, using TA, Blunt or Directional methods.

Start Tutorial >