LEARN GENEIOUS

Getting Started with Geneious Prime

Learning how to use Geneious Prime is easy! We developed a few short videos to help guide you through the process. From how to import your data to aligning your first sequences, follow these tutorials and you will master the basics in no time.

How To Import and Organize Your Files

INSIDE THE VIDEO
Learn how to import new files, organize the folder system and search for documents.

LEARN HOW TO:

  • Store files in local and shared databases
  • Create, move and customize folders
  • Import sequences from NCBI and UniProt databases
  • Search and filter files within Geneious Prime

How To View Documents and Sequences

INSIDE THE VIDEO
Overview of the viewing, annotation and customization options for sequences and documents.

LEARN HOW TO:

  • View list of files in the document table
  • Identify different file types
  • Customize view using tabs in the control panel
  • Zoom, annotate, split the view and change the colors of your sequences

How To Perform Analyses in Geneious Prime

INSIDE THE VIDEO
Get started running analyses, or operations, with simple sequence selection and the quick access toolbar.

LEARN HOW TO:

  • Run analyses such as BLAST, alignment, primer design and cloning
  • Choose your method or algorithm
  • Adjust settings for your analysis
  • Find more information on each tool

How To Annotate Sequences

INSIDE THE VIDEO
Customize the viewing of your annotations and create new annotations from existing files or databases.

LEARN HOW TO:

  • Display annotations on your sequence or in a table
  • Import annotation files
  • Manually add annotations
  • Automatically add annotations from the reference feature database
  • Transfer annotations from a similar sequence

How To Find and Analyze Restriction Enzymes

INSIDE THE VIDEO
Find, add, remove and analyze restriction sites and the resulting fragments.

LEARN HOW TO:

  • Select enzymes by enzyme set or characteristics
  • Display a table of all enzymes and information
  • Simulate an agarose gel using chosen restriction enzymes
  • View and export fragments information
  • Create your own custom enzyme set

How To Simulate Restriction Cloning

INSIDE THE VIDEO
Simulate restriction cloning of a single insert into a vector backbone.

LEARN HOW TO:

  • Select a vector and insert for restriction cloning
  • Select enzymes from annotated enzymes, an enzyme set or from the enzyme drop down menu
  • Add additional inserts and rearrange inserts
  • View the final cloning product including annotations and concatenated sequence

How To Design Primers

INSIDE THE VIDEO
View, design and export primers for PCR, cloning or sequencing.

LEARN HOW TO:

  • Automatically design new primers
  • Manually create primers directly from your sequence
  • Extract PCR product from a primer pair
  • Adjust melting temperature, length, hairpin and GC content settings
  • View primers and characteristics on your sequence and in tables

How To Align Chromatograms to a Reference Sequence

INSIDE THE VIDEO
Align your chromatograms to a reference sequence so you can find variants and verify sequences.

LEARN HOW TO:

  • Prepare your data including checking sequence quality and trimming the ends
  • Map a chromatogram sequence against a reference sequence
  • View the contig, change your display and edit bases