Efficient and accurate sequence assembly with MIRA
Requires Geneious 7.1+
The MIRA (Mimicking Intelligent Read Assembly) genome fragment assembler is great for ‘tricky’ projects with high numbers of repeats and it can handle hybrid data. MIRA is used for tasks such as mutation detection in different cell types, similarity analysis of transcripts between organisms, and pristine assembly of sequences from various sources for oligo design in clinical microarray experiments. It works best on sequences with up to 100-150 megabases or 20-40 million reads.
Install the plugin by downloading the gplugin file and dragging it in to Geneious or use the plugin manager in Geneious (under Tools - Plugins in the menu).
Once installed, run the plugin by selecting your sequencing reads and clicking on Align/Assemble - De Novo Assemble in the toolbar. MIRA is available under the Algorithm drop-down option.
1.0.1 (18 Mar 2014)
- Fix hangs when assembly report contains too much text (which happens on big MIRA assemblies)
- MIRA doesn't handle RNA, translate to DNA before passing data to MIRA
- Fix for space character in executable path producing error messages
- MacOS: enable use of tcmalloc to reduce memory usage
- MacOS: static link stdc++, removing the bundled shared library libstdc++
Chevreux et al. (2004) Using the miraEST Assembler for Reliable and Automated mRNA Transcript Assembly and SNP Detection in Sequenced ESTs, Genome Research 2004. 14:1147-1159.