One-step Multicistronic Cloning

Learn how to perform one-step multicistronic or bicistronic cloning using Gibson Assembly or Golden Gate Cloning.

How to Perform One-step Multicistronic Cloning

INSIDE THE VIDEO
Learn how to perform one-step multicistronic or bicistronic cloning using Geneious Prime. Work with variable light regions, heavy regions, and backbone sequences using both Gibson Assembly and GoldenGate methods.
LENGTH
4 minutes

Recommended Resources

Introduction to the cloning interface

Cloning tools share a common interface which differs only for specific options.

How do I add primers to Geneious Prime?

Add existing primers to Geneious manually or by importing from external sources.

Manual for Gibson Assembly

A guide to using the Gibson assembly tool in Geneious.

Geneious Academy

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Watch the series to learn the basics of Golden Gate cloning and how to simulate standard or batch Golden Gate cloning.
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Use the Transfer Annotations tool to transfer features to a reference or consensus sequence in an alignment or assembly.
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Group reads into clones, map clones to a reference and automate validation of sequences with the cloning validation tool.
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