What’s New in Geneious Prime


Geneious Prime 2024.0

Geneious Prime 2024.0 brings new features and functionality which include Cloning Validation improvements, a Map to Reference algorithm advisor, and De Novo Assembly improvements.

Released 02/2023  |   Geneious Prime 2024.0 Full Release Notes

Geneious Prime 2024.0 Feature Overview

Watch for an overview of the new features in Geneious Prime 2024.0

Cloning Validation

New visual and semi-automated step to add, export and record validation metadata during batch plasmid validation.

Map to Reference Algorithm Advisor

Interactive quiz to help you decide which algorithm is the best fit for you data.

Previous Releases

Geneious Prime 2023

Geneious Prime 2023 brings powerful analyses, a more user-friendly interface and improved efficiency for high throughput cloning workflows. Highlights also include Geneious Cloud Personal Workspace, the De Novo Assembly Algorithm Adviser, improvements to STAR RNA-seq aligner, the ability to export lengths graph data, CRISPR improvements, Cloning Validation visual tools, primer specificity testing and the Dotmatics Platform Integration Plugin. 

Geneious Prime 2023 Full Release Notes

Geneious Prime 2023.0 Feature Overview

Geneious Prime 2023.2 Feature Overview

De Novo Assembly Algorithm Advisor

Interactive quiz to help you decide which algorithm is the best fit for your data.

Cloud Personal Workspace

Access your data from multiple computers with secure personal data storage in Geneious Prime.

Geneious Cloud Database is currently available to academic customers with a personal subscription. 

Learn More >

Export Lengths Graph Data

Quickly and easily export a csv file with your sequence length distribution data.

Improvements to STAR RNA-Seq Aligner

You can now use the STAR RNA-seq aligner in Geneious with multiple references.

Dotmatics Platform Integration Plugin

Launch the registration workflow to register your sequences in Dotmatics Bioregister

Import .sbd files

Geneious Prime can now import DNAStar Lasergene SeqBuilder(Pro) .sbd file format.

Primer Specificity Testing

Design target-specific primers in one step with new primer specificity testing.

Cloning Validation

New visual tool for automating sequence alignment during batch plasmid validation

STAR RNA-seq mapper

Added STAR, a RNA-seq mapper that performs highly accurate spliced sequence alignment.

CRISPR Improvements

Added more PAMs and the ability to select individual sequences for specificity testing.

Geneious Prime 2022

Geneious Prime 2022 brings powerful analyses, a more user-friendly interface and improved efficiency for high throughput cloning workflows. Highlights include In-Fusion cloning, annotating by BLAST, a command line interface, ability to apply and revert optimized codons, dark mode, parts cloning support for proteins, batch Golden Gate cloning and primer extension detection.

Geneious Prime 2022 Full Release Notes

Geneious Prime 2022.0 Feature Overview

Geneious Prime 2022.1 Feature Overview

Geneious Prime 2022.2 Feature Overview

Command Line Interface
Run Geneious Prime operations and workflows as part of your broader bioinformatic pipelines locally or execute on more powerful servers.
Learn how to run Geneious operations via the command line

In-Fusion Cloning
Perform low or high throughput In-Fusion Cloning with pre-optimized settings.

Parts Cloning Support for Proteins
Scale up your construct engineering with combinatorial assembly of proteins.

Primer Extension Detection
Automatically detect and annotate extensions on your primer databases during import.

Annotate by BLAST
Automatically annotate your ORFs, CDS and mRNA regions using BLAST.
Learn how to use Annotate by BLAST to annotate nucleotide sequences

Manage Document Table Columns
Quickly organize your data with multiple column selection

Highlight Mismatching Bases
Highlight mismatching base calls in your chromatograms

Experimental Features
Try out features we are experimenting with and let us know your thoughts

Apply Optimized Codon
Easily add and remove optimized codons to your sequence

Batch Golden Gate
Perform Golden Gate cloning in batch using sequence lists and stop doing your Golden Gate cloning operations one by one.

Primer Import From Excel Spreadsheets
Easily import and characterize primer databases stored in excel spreadsheets so you can take full advantage of the primer tool suite, including Test with Saved Primers.

Parts Cloning
Scale up your cloning throughput with added support for Parts Cloning, combining every possible combination from your lists of parts.

Dark Mode
Comfortably analyze your data at any time of the day or night with Geneious Prime in dark mode (beta)

Geneious Prime 2021

Geneious Prime 2021 brings a new look and easier search, to make sequence analysis more intuitive and user-friendly. Highlights include a redesigned user interface, virtual gel improvements, CRISPR cut sites and on-target scoring method,  updated search and filters and adjustable sequence selection.

Geneious Prime 2021 Full Release Notes

Geneious Prime 2021.0 Feature Overview

Geneious Prime 2021.1 Feature Overview

Geneious Prime 2021.2 Feature Overview

Geneious Prime gets a new look
Enjoy the new look and feel of Geneious Prime with an updated design, including new icons and font.

New CRISPR on-target scoring method
Confidently design your guide RNAs using the Doench et al. 2016 CRISPR on-target scoring algorithm.

Virtual gel improvements
Easily view multiple sequences with the display of restriction enzymes in lane headings, and quick access to an exportable list of fragments

Accessible sequence viewer statistics
Easily sort and export your protein and nucleotide statistics within the document table.

Highlighted methylation sites for restriction enzymes
Quickly visualize restriction sites affected by Dam, Dcm and EcoKI methylation.

Adjustable sequence selection
Adjust either end of the selected region in the Sequence View by dragging.

Amino acid numbering
Live numbering can now be shown alongside the translated amino acids on nucleotide sequences.

New filter interface
Locate documents quickly in any table with the new filter button added to the status bar.

View CRISPR cut sites
Easily view and compare CRISPR cut sites between your guide RNAs

View invalid CDS annotations
Improve your cloning workflows by quickly determining CDS annotations with premature stop codons

Batch edit annotations
Quickly add or edit annotations in batch by copying properties from other annotations, sequences or documents

Geneious Prime 2020

Geneious Prime 2020 brings exciting new functionality to transform raw sequence data into meaningful visualizations. Highlights include long-read sequence mapping, codon optimization, a wrapper plugin system, CRISPR editing analysis and updates to annotate from database.

Geneious Prime 2020 Full Release Notes

Long-read sequence mapping with Minimap2 (external plugin)
Fast and easy alignment of Oxford Nanopore and PacBio data to a reference sequence using an industry recommended tool, without the hassle of the command line

Clustal Omega replaces ClustalW for better, faster alignments
Scale up your alignments with Clustal Omega, now included with Geneious Prime. The HMM alignment engine improves both quality and speed of alignments compared to the older ClustalW aligner.

Better back translate and codon optimization
New codon optimization algorithm lets you match the codon usage from your organism of choice by generating a new sequence, starting from either a protein or nucleotide sequence. Optionally eliminate rare codons and restriction sites.

More accurate annotation of CDS features onto your plasmids
Automatic annotation of plasmids is now smarter with adjustment of CDS boundaries to match the nearest valid ORF when annotating single-interval CDSs from your reference features database.

Add your favorite tools to Geneious with improved Wrapper Plugin system
Can’t wait for us to add your favorite command line aligner, tree builder or assembler? You might be able to add it yourself from right inside Geneious using the Wrapper Plugin Creator plugin. Coding skills are not required, though a good understanding of command line interfaces and file formats is.

Long-read de novo Assembly with Flye (external plugin)
Fast de novo assembly of your raw Oxford Nanopore and PacBio long read sequences using Flye, suited to a wide range of applications including both single genome projects and metagenome assembly.

Import Metadata on to Sequences and other Documents
Seamlessly attach new data from downstream analyses or other applications onto your sequences in Geneious, or update document fields, by importing columns from a CSV/TSV format spreadsheet onto documents that are already in Geneious.

Annotate from Database Improvements
Easily identify and annotate the best matched features from a database on your plasmids and other sequences using the new extended database of standard plasmid features provided with Geneious, or use your own sequences as reference.

Codon Optimization and Back Translation Improvements
Customize the codon optimization parameters for your model organism by defining short sequence motifs to exclude from your optimized sequence, while easily generating multiple co-optimized versions of your sequence simultaneously.

Primer Specificity Testing
Be confident your primers will only bind in a single location with automatic identification of any additional, off target, binding sites found when using Test with Saved Primers. Off-target information for the tested document will be added to the new primer annotations.

Wrapper Plugin System Improvements
Customize Geneious with your own plugins using the wrapper plugin system, which now supports de novo assemblers, and running supported linux-only tools via the Windows Linux Subsystem.

Analyze CRISPR Editing Results
Easily align, cluster and visualize NGS reads from your CRISPR editing experiments. Analyze the frequency of variants and their protein effects from your application of choice, including HDR, NHEJ and base editors.

Enhanced Search Options
Quickly access your documents, folders, analysis tools and recently viewed items from a single unified interface.

Codon Optimization Improvements
Hover over your optimized codons for more information on synonymous codons and their frequencies.

Geneious Prime 2019

Geneious Prime 2019 brings powerful analyses to increase accuracy and help transform collaboration. Highlights include updates to primer design, custom codon usage, import and export, annealed oligo cloning, restriction enzymes and shared database.

Geneious Prime 2019 Full Release Notes

Custom Codon Usage Tables
Back translate and optimize codons using your own codon usage tables. Import standard formats from public databases or external codon analysis tools.

Rapid Manual Primer Design
Select desired binding site in the Sequence View to see real-time display of length and melting point (Tm) then easily add a primer annotation with a convenient new button.

Powerful Primer Annotation Display
5′ extensions (tails) on annotated primers are now displayed inside the sequence view to indicate the length of the extension, the nucleotides it contains and what functional elements are present such as restriction sites, spacers and tags.

Simplified Testing and Annotation of Existing Primers
Easily search all folders for primers that match a sequence of interest with the redesigned “Test with Saved Primers” operation. Copy and paste primers from other programs into the new “Add Primers” operation to find them on your target sequence in one step.

Drag and Drop Sequence Export
Export annotated sequences in GenBank format by simply dragging documents out of Geneious Prime and onto the desktop or into another program.

Easy Extraction of PCR and Restriction Digest Products
Shift click between two compatible primers or restriction enzymes for quick and easy extraction of their product from your sequence.

Improved Protein Statistics
Calculate on the fly statistics for protein sequences directly on nucleotide sequences within the Statistics panel, with new protein statistics added including charge at pH 7 and amino acid group counts.

Improved GenBank Export
Export the information you need with your sequences using the simplified, flexible options to include your own Meta-Data fields and allow or constrain export parameters to suit downstream applications.

Publish Plugins via Internal Network
Create a curated list of plugins to offer privately to members of your organization via your internal network.

Customizable Text View
GenBank format available for all individual sequences. Sequence layout format to alter wrapping, numbering, sequence color and presence of reverse complement and translation. Alignment layout to alter numbering, sequence color and highlighting of agreements/disagreements to consensus.

Primer Specificity
Primer specificity automatically tested within the template sequence when designing primers, and presence of any additional binding sites recorded on the primer annotation

Primer Design Improvements
Option to control the minimum distance between primers designed on the same strand – defaults to minimizing creation of duplicate primers when multiple primer pairs are designed together. Quick and easy primer pairing in the Sequence View to link two primers of your choice and calculate pair dimer Tm and product size.

Vector NTI Database Import
Easily import your complete Vector NTI database with simple drag and drop, for both Vector NTI Express and Advance databases.

Restriction Enzymes
Methylation sensitivity information shown in tool tip when mousing over a restriction enzyme

Sanger Sequencing Primer Design
Quickly design primers for Sanger sequencing of vectors, inserts or other sequences

Easier Shared Database Rollout
Immediately access shared data and get straight to doing your research with a Geneious Shared database connections now able to be pre-configured by your IT team or admin when rolling out a version of Geneious Prime. Includes improved support for Windows authentication.

Improved Text View Formatting of Sequences and Alignments
Support formatting and export of sequences in accordance with U.S. Patent Office requirements as well as other improvements.