Previous Versions of Geneious Prime

History and download links for all of the major releases of Geneious Prime

Geneious Prime 2023

2023 Full Release
30 November 2022
2023 release notes
Primer Specificity Testing
Design target-specific primers in one step with new primer specificity testing.
Cloning Validation
New visual tool for automating sequence alignment during batch plasmid validation
STAR RNA-seq mapper
Added STAR, a RNA-seq mapper that performs highly accurate spliced sequence alignment.
CRISPR Improvements
Added more PAMs and the ability to select individual sequences for specificity testing.
Batch export multiple image files
Export multiple files with ease
Cloning UX
Consistency across ops and fixed primer duplication
General UX
New progress display and stamp
2023.1 Update
04 April 2023
2023.1 release notes
Dotmatics Platform Integration plugin
Launch the registration workflow to register your sequences in Dotmatics Bioregister.
Import .sbd files
Geneious Prime can now import DNAStar Lasergene SeqBuilder(Pro) .sbd file format.
Geneious Cloud Database (beta)-now bundled
Access your data from multiple computers with secure personal data storage in Geneious Prime.
2023.2 Update
25 July 2023
2023.2 release notes
De Novo Assembly Algorithm Advisor
nteractive quiz to help you decide which algorithm is the best fit for your data.
Cloud Personal Workspace
Access your data from multiple computers with secure personal data storage in Geneious Prime.
Export Lengths Graph Data
Quickly and easily export a csv file with your sequence length distribution data.
Improvements to STAR RNA-seq aligner
You can now use the STAR RNA-seq aligner in Geneious with multiple references.

Geneious Prime 2022

16 November 2021
2022 release notes
Annotate by BLAST
Automatically annotate your ORFs, CDS and mRNA regions using BLAST
Dark Mode
Comfortably analyze your data at any time of the day or night with Geneious Prime in dark mode (beta)
Command Line Interface
Run Geneious Prime operations and workflows as part of your broader bioinformatic pipelines locally or execute on more powerful servers
Apply Optimized Codon
Easily add and remove optimized codons to your sequence
Manage Document Table Columns
Quickly organize your data with multiple column selection
Highlight Mismatching Bases
Highlight mismatching base calls in your chromatograms
Experimental Features
Try out features we are experimenting with and let us know your thoughts
2022.1 Update
16 March 2022
2022.1 release notes
Parts cloning
Scale up your cloning throughput with added support for Parts Cloning, combining every possible combination from your lists of parts.
Batch Golden Gate cloning
Perform Golden Gate cloning in batch using sequence lists and stop doing your Golden Gate cloning operations one by one.
Primer import from Excel spreadsheets
Easily import and characterize primer databases stored in excel spreadsheets so you can take full advantage of the primer tool suite, including Test with Saved Primers.
2022.2 Update
12 July 2022
2022.2 release notes
Command Line Interface
Sync data from your pipelines directly into (or out of) your Geneious Prime local or shared databases.
In-Fusion Cloning
Perform low or high throughput In-Fusion Cloning with pre-optimized settings.
Parts Cloning support for proteins
Scale up your construct engineering with combinatorial assembly of proteins.
Primer extension detection
Automatically detect and annotate extensions on your primer databases during import.

Geneious Prime 2021

1 December 2020
2021 release notes
Geneious Prime gets a new look
Enjoy the new look and feel of Geneious Prime with an updated design, including new icons and font.
New CRISPR on-target scoring method
Confidently design your guide RNAs using the Doench et al. 2016 CRISPR on-target scoring algorithm.
Accessible sequence viewer statistics
Easily sort and export your protein and nucleotide statistics within the document table.
Highlighted methylation sites for restriction enzymes
Quickly visualize restriction sites affected by Dam, Dcm and EcoKI methylation.
2021.1 Update
16 March 2021
2021.1 release notes
New filter interface
Locate documents quickly in any table with the new filter button added to the status bar.
Amino acid numbering
Live numbering can now be shown alongside the translated amino acids on nucleotide sequences.
Adjustable sequence selection
Adjust either end of the selected region in the Sequence View by dragging.
2021.2 Update
13 July 2021
2021.2 release notes
Virtual gel improvements
Easily view multiple sequences with display of restriction enzymes in lane headings, and quick access to an exportable list of fragments.
View CRISPR cut sites
Easily view and compare CRISPR cut sites between your guide RNAs.
View invalid CDS annotations
Improve your cloning workflows by quickly determining CDS annotations with premature stop codons.
Batch edit annotations
Quickly add or edit annotations in batch by copying properties from other annotations, sequences or documents.

Geneious Prime 2020

5 November 2019
2020 release notes
Longread sequence mapping with Minimap2 (external plugin)
Fast and easy alignment of Oxford Nanopore and PacBio data to a reference sequence using an industry recommended tool, without the hassle of the command line
Clustal Omega replaces ClustalW for better, faster alignments
Scale up your alignments with Clustal Omega, now included with Geneious Prime. The HMM alignment engine improves both quality and speed of alignments compared to the older ClustalW aligner.
Better back translate and codon optimization
New codon optimization algorithm lets you match the codon usage from your organism of choice by generating a new sequence, starting from either a protein or nucleotide sequence. Optionally eliminate rare codons and restriction sites.
More accurate annotation of CDS features onto your plasmids
Automatic annotation of plasmids is now smarter with adjustment of CDS boundaries to match the nearest valid ORF when annotating single-interval CDSs from your reference features database.
Add your favorite tools to Geneious with improved Wrapper Plugin system
Can’t wait for us to add your favorite command line aligner, tree builder or assembler? You might be able to add it yourself from right inside Geneious using the Wrapper Plugin Creator plugin. Coding skills are not required, though a good understanding of command line interfaces and file formats is.
2020.1 Update
10 March 2020
2020.1 release notes
Long Read de novo Assembly with Flye (external plugin)
Fast de novo assembly of your raw Oxford Nanopore and PacBio long read sequences using Flye, suited to a wide range of applications including both single genome projects and metagenome assembly.
Import Metadata on to Sequences and other Documents
Seamlessly attach new data from downstream analyses or other applications onto your sequences in Geneious, or update document fields, by importing columns from a CSV/TSV format spreadsheet onto documents that are already in Geneious.
Annotate from Database Improvements
Easily identify and annotate the best matched features from a database on your plasmids and other sequences using the new extended database of standard plasmid features provided with Geneious, or use your own sequences as reference.
Codon Optimization and Back Translation Improvements
Customize the codon optimization parameters for your model organism by defining short sequence motifs to exclude from your optimized sequence, while easily generating multiple co-optimized versions of your sequence simultaneously.
Primer Specificity Testing
Be confident your primers will only bind in a single location with automatic identification of any additional, off target, binding sites found when using Test with Saved Primers. Off-target information for the tested document will be added to the new primer annotations.
Wrapper Plugin System Improvements
Customize Geneious with your own plugins using the wrapper plugin system, which now supports de novo assemblers, and running supported linux-only tools via the Windows Linux Subsystem.
2020.2 Update
14 July 2020
2020.2 release notes
Analyze CRISPR Editing Results
Easily align, cluster and visualize NGS reads from your CRISPR editing experiments. Analyze the frequency of variants and their protein effects from your application of choice, including HDR, NHEJ and base editors.
Enhanced Search Options
Quickly access your documents, folders, analysis tools and recently viewed items from a single unified interface.
Codon Optimization Improvements
Hover over your optimized codons for more information on synonymous codons and their frequencies.

Geneious Prime 2019

6 November 2018
2019 release notes
Custom Codon Usage Tables
Back translate and optimize codons using your own codon usage tables. Import standard formats from public databases or external codon analysis tools.
Rapid Manual Primer Design
Select desired binding site in the Sequence View to see real-time display of length and melting point (Tm) then easily add a primer annotation with a convenient new button.
Powerful Primer Annotation Display
5′ extensions (tails) on annotated primers are now displayed inside the sequence view to indicate the length of the extension, the nucleotides it contains and what functional elements are present such as restriction sites, spacers and tags.
Simplified Testing and Annotation of Existing Primers
Easily search all folders for primers that match a sequence of interest with the redesigned “Test with Saved Primers” operation. Copy and paste primers from other programs into the new “Add Primers” operation to find them on your target sequence in one step.
Drag and Drop Sequence Export
Export annotated sequences in GenBank format by simply dragging documents out of Geneious Prime and onto the desktop or into another program.
Easy Extraction of PCR and Restriction Digest Products
Shift click between two compatible primers or restriction enzymes for quick and easy extraction of their product from your sequence.
Improved Protein Statistics
Calculate on the fly statistics for protein sequences directly on nucleotide sequences within the Statistics panel, with new protein statistics added including charge at pH 7 and amino acid group counts.
Improved GenBank Export
Export the information you need with your sequences using the simplified, flexible options to include your own Meta-Data fields and allow or constrain export parameters to suit downstream applications.
Publish Plugins via Internal Network
Create a curated list of plugins to offer privately to members of your organization via your internal network.
Usability Tweaks to Increase Efficiency
Enjoy an improved table of primers, drag and drop backbone selection for cloning, easier workflow management, better defaults, a less cluttered Sequence View and many other little tweaks and improvements.
2019.1 Update
5 March 2019
2019.1 release notes
Customizable Text View – Sequence Layout 
Format to alter wrapping, numbering, sequence color and presence of reverse complement and translation
Customizable Text View – Alignment Layout
Format to alter numbering, sequence color and highlighting of agreements/disagreements to consensus
Customizable Text View – GenBank Format
GenBank format available for all individual sequences
Primer Specificity
Primer specificity automatically tested within the template sequence when designing primers, and presence of any additional binding sites recorded on the primer annotation
Primer Design Improvements
Option to control the minimum distance between primers designed on the same strand – defaults to minimizing creation of duplicate primers when multiple primer pairs are designed together
Annealed Oligo Cloning
Added workflow to create a sequence with annotated overhangs representing the sticky ends created by annealing two partially overlapping oligos
Restriction Enzymes
Methylation sensitivity information shown in tool tip when mousing over a restriction enzyme
2019.2 Update
18 June 2019
2019.2 release notes
Sanger Sequencing Primer Design
Quickly design primers for Sanger sequencing of vectors, inserts or other sequences
Vector NTI Database Import
Easily import your complete Vector NTI database with simple drag and drop, for both Vector NTI Express and Advance databases.
Improved Text View Formatting of Sequences and Alignments
Support formatting and export of sequences in accordance with U.S. Patent Office requirements as well as other improvements.
Primer Characteristics
Quick and easy primer pairing in the Sequence View to link two primers of your choice and calculate pair dimer Tm and product size.
Easier Shared Database Rollout
Immediately access shared data and get straight to doing your research with a Geneious Shared database connections now able to be pre-configured by your IT team or admin when rolling out a version of Geneious Prime. Includes improved support for Windows authentication.


3 October 2017
11.0 release notes
Volcano Plots for RNA-Seq Expression Analysis
Visualize gene expression in an interactive volcano plot that can be used to highlight and jump to differentially expressed genes.
Pretty PCA Plots
Create beautiful PCA plots with better labelling and new visualization options.
Silent Mutation Analysis for Restriction Sites
Automatically identify point mutations in a coding sequence that will introduce a restriction site without affecting the protein.
CRISPR Cpf1 Option
Added support for CRISPR-Cpf1 with a 5’ PAM site. Find sites in A and T rich sequences and with higher cleaving efficiency in vivo.
Create Enzyme Sets
What’s in the freezer? No longer pull information from other sources. Now you can easily create your own enzyme sets.
Better FASTQ Import
Paired reads can be associated with each other during import and Geneious will do it’s best to guess how they’re paired. Read technology can also be set.
Updated Mauve Plugin for Bacterial Genome Alignment
Now includes support for sequence lists and the MCM operation for reordering contigs, making it possible to align draft genomes and perform genome finishing.
R11.1 Update
6 March 2018
R11.1 release notes
SnapGene File Import
Drop SnapGene *.dna sequence files into Geneious to seamlessly import sequences along with their annotations and other metadata.
CRISPR-Cpf1 Specificity Scoring
Confidently pick the best CRISPR-cpf1 guide RNA sites using a new specificity score which is calculated from an off-target analysis against your database of choice.
Smart NGS Import
Drop any assortment of SAM, BAM, GFF, BED, and VCF files into Geneious to import in one easy step, even if you have a mixture of different samples and reference sequences.
Better BLAST Server Management
Set up several BLAST server mirrors at once and choose the best one each time you do a search. No more switching mirrors each time.
NCBI BLAST Cloud Support
It’s never been easier to set up your own BLAST mirror thanks to NCBI BLAST Cloud. If you set up a mirror in this way, Geneious can now connect to it natively.
Concatenate by Index
Concatenate sequence lists and alignments by index, rather than by name, which is super useful for merging paired reads that don’t quite overlap.
CSV/TSV Export of Sequence Lists
Export all of the sequences in a sequence list along with their metadata to CSV or TSV so you can drop them in Excel or similar for further analysis.


4 October 2016
10.0 release notes
Smarter Restriction Cloning
Completely redesigned to be much simpler and more powerful. Includes automatic identification of compatible cut sites, and digestion and ligation with drag and drop ordering of fragments.
Alignment Masking
Mask unreliable alignment sites for better tree building, without deleting data. Manage alignment masking patterns using annotation tracks and automatically mask sites according to user defined criteria.
Improved Plasmid Viewer
The new circular overview option helps you stay oriented by showing the bases of the sequence and the plasmid map alongside each other with synchronized editing, selection and scrolling.
Chimera Filtering
Filter chimeric reads by comparing to a reference database, which can be any sequence list or alignment. Choose between the bundled public domain UCHIME algorithm or the faster USEARCH implementation.
Lineage View for Parent/Descendants
Lineage View is now easier to access as a separate tab and details about your cloning operations are shown right next to the relationships.
Assembly and Mapping
Better structural variant mapping, a new workflow to build SNP trees by applying variants to a reference and better de novo assembly of Ion Torrent, 454, and PacBio CCS data.
R10.1 Update
7 February 2017
R10.1 release notes
Improved Cloning
Gibson and Golden Gate now has sequence view of fragments within the options. Gibson assembly can now saves primers for easy ordering
Annotate from Database
Annotate nucleotide sequences from a protein database by comparing translations of the nucleotide sequence in all six frames with the protein database sequences
Zip Import
Zip files containing multiple files and sub-folders can now be imported
Sequence Viewer
Added ‘Copy Translation’ to translate selected regions of a nucleotide sequence (in right-click menu)
SPAdes de novo assembler
For MacOS/Linux: 64 bit OS is required. For Windows, 64 bit Windows 10 (with recent updates installed) is required. You also need to install additional Windows features (Instructions)
Find CRISPR Sites
Major performance improvements were made to the off-target scoring algorithm
All CRISPR sites will now be scored against all potential off-target sites in every run
Scoring CRISPR sites against off-targets now takes a seed region into account. This is a 10 bp region adjacent to the PAM site that can tolerate a maximum of 2 mismatches and 0 indels
R10.2 Update
6 June 2017
R10.2 release notes
RNA-Seq Expression Analysis
Compare Expression Levels now has the option to use DESeq2 for pairwise analysis with replicate samples and will produce a principle component analysis (PCA) plot for quality control.
Cloning Workflows
Restriction Cloning, Gibson Assembly and Golden Gate can now be incorporated into Workflows. Automate your cloning procedures or perform high-throughput batch cloning.


7 October 2015
9.0 release notes
Golden Gate
Intelligent handling of preconfigured Golden Gate parts and automatic designing of primers for new parts.
RNA- Seq and Structural Variant Mapping
Map RNA-seq using existing annotated coding regions or discover novel intron and fusion genes during mapping. For DNA sequencing, you can now discover structural variants and annotate them, allowing for correct alignments of read-ends around deletions and structural rearrangements in heterozygous samples.
Big Trees
New search box for finding nodes and taxa, and automatic collapsing of sub-trees based on distances. Greatly improved performance when loading and rendering trees.
Cutting edge CRISPR
New scoring strategy for on-target activity. Options to control how many mismatches and indels are allowed when scoring against off-targets.
VCF Export
Easy exporting of single-sample variants.
Brand new and super-fast de novo assembler with a very low miss-assembly rate.
Primer Design
Improved the speed on larger sequences when a target region and product size range have been specified
Read filtering with built-in BBTools

  • Merge paired reads using BBMerge
  • Error correction and coverage level normalization using BBNorm
  • Duplicate read removal using BBDedupe
  • BBDuk plugin for additional trimming (provides adapter, paired read overlap, quality, minimum length, and entropy trimming)
New Plugins

  • BBMap mapper for DNA/RNAseq
  • EupathDB for searching and retrieving eukaryotic pathogen sequences
  • Repeat Finder for annotating all regions that are repeated elsewhere in a sequence
R9 Update
1 March 2016
R9.1 release notes
Even better support for HiDPI displays on Windows and Linux. Most importantly, the sequence and alignment view is now scaled according to your display.
Alignment and contig viewing
redesigned “go to next” controls give more power in a more intuitive interface.
Sequence view
Updated to new modern font for high quality publications, cleaner plasmid maps and retina screens.
Gibson Cloning
now fully supports the full array of scarless methods including SLIC, SLiCE, CPEC, InFusion and GeneArt Seamless.
New workflow to run batch reference mapping of many data sets against many references, matching them up by name.
Sequences in the multiple alignment are now highlighted according to coloring in the tree.
Parents and descendants are now shown with more information about the steps that have been performed and an HTML report can be exported with the full lineage.


9 September 2014
8.0 release notes
16S Biodiversity Tool
A cloud-based tool that identifies high-throughput 16S rRNA amplicons from environmental samples using the RDP database, and visualizes biodiversity as interactive graphs and charts using a secure web viewer.
Sequence Classifier Plugin
Taxonomically classifies an organic sample by how similar its DNA is to your own database of known sequences using a BLAST-like algorithm with multiple loci and trees to assist with identification.
Locate potential CRISPR target sites using innovative heatmap-style annotations to assess sites based on off-target interaction.
Powerful NGS Assembly
Longer contigs, scaffolding and greater accuracy.
RNA-Seq Expression Analysis
Brand new functionality for transcriptomics!
Greater Workflow Configurability
New Genome finishing workflow and more options for creating custom workflows
Easier Plugin Development
Add your favourite algorithm, database or visualization to Geneious or write a plugin for your own new program and share it with the community
New Plugins
Extended functionality for tree building and viewing, alignment and assembly.
CRT – (by Chris Duran) Identifies existing known CRISPR sites in bacteria and archaea.
FLASH – (by Dave O’Connor)- Merge paired sequencing reads using FLASH
SeqPartitioner – (by Jed Barlow) – Partition allele multisets from multiple alignments
Augustus – (by Michael Thon) Predict genes using the Augustus algorithm.
EMBOSS Nucleotide Analysis – (previously bundled) Search for transcription factors, and predict protein coding regions.
EMBOSS Protein Analysis – (previously bundled) Predict secondary structure, antigenic regions and signal cleavage sites.
Go To Documents – Jump to documents by entering their unique IDs
Find Big Folders Workflow – List the size of all folders in your local database
Groovy Console – Opens a terminal window inside Geneious for scripting in the Groovy language.
R8 Update
3 March, 2015
8.1 release notes
Choicer chooser
Store commonly used sequences such as reference genomes, vectors and biological parts in separate folders from your working data, then easily choose them when you run Map to Reference or Gibson Assembly.
Sort by GC Content
All newly created nucleotide alignments, contigs, sequence lists, and sequences have a %GC field in the document table. Sequences in alignments, contigs and lists can be sorted by %GC.
Improved Primer Design for qPCR
Batch design primers for many regions within a single sequence in one step, great for qPCR design. Primer design and testing now have an option to match each sequence in an alignment (instead of just the consensus)
Extract more annotation information
Now you can select upstream, downstream and intergenic regions of matching annotations.
Mapping Qualities now included
Geneious mapper produces mapping qualities, SAM/BAM import/export handles mapping qualities, contig viewer displays mapping quality in status bar, added mapping quality color scheme
Integration with LIMS and external web-based systems
Geneious documents can now be opened by clicking a link in external web-based systems.
Easier Exporting for Newick and FASTA formats
Documents can now be exported in the compressed .fasta.gz and .fastq.gz formats. Export Tree documents: Export to newick format may now include bootstrap support values
New Plugins
Blast2GO – All in one functional annotation tool and annotation data analysis.
FreeBayes – Haplotype-based variant detector for single sample analysis.


3 September 2013
7.0 release notes
Easily create and share workflows based on your data and analysis. Try some of the built-in workflows or design your own.
Gibson Assembly
One-step cloning of fragments using Gibson assembly including automated primer design and batch cloning/shuffling
Codon Optimization
Find rare codons or produce a fully optimized sequence based on a target organism and remove unwanted restriction sites at the same time.
TOPO Cloning
One-step cloning of fragments into a vector using TOPO
Copy and Paste Annotations
Annotations are now maintained when copying sequences! Highlight part of a sequence, copy with ctrl+c and then paste it using ctrl+v into another location and the annotations will be preserved.
Better Coverage Analysis
Get more meaning from your alignments with added visualization of coverage.
New Plugins
Extended functionality for tree building and viewing, alignment and assembly.
RAxML – Randomized Axelerated Maximum Likelihood
GARLI – Genetic Algorithm for Rapid Likelihood Inference
FastTree – Approximately-maximum-likelihood phylogenetic trees from alignments of nucleotide or protein sequences
LastZ – BLAST-like alignment tool for the pairwise alignment of chromosome-sized nucleotide sequences
Bowtie2 – Short read aligner
Velvet – De-novo assembler for very short reads
R7 Update
4 February 2014
7.1 release notes
Circular Assembly
Geneious can create circular contigs in its famous sequence viewer making it easier to assemble genomes such as bacterial genomes and plasmids.
Trio Analysis
The improved ‘compare annotation’ interface gives better results and more powerful options for SNP analysis and all results include annotation properties.
Strip Alignment columns
Build better trees! Remove columns with at least one ambiguity or strip two columns per codon.
Extract more annotation information
Now you can select upstream, downstream and intergenic regions of matching annotations.
New Plugins
TopHat – Fast splice junction mapper for RNA-Seq reads.
MIRA – Specialized assembler for sequences with a high number of repeats


4 October 2012
6.0 release notes
Latest Primer3 engine
Including brand new thermodynamic calculations that give you better stats on primers.
Automatic plasmid annotation
No more manual searching. New automatic plasmid annotation lets you sort through your data faster than ever before, saving your time and your eyesight.
Read Mapping
New fine-tuning algorithm quickly generates accurate results using less memory.
Enhanced Variant and SNP calling
Eliminate false variants, annotate average quality, find variations within specified annotations. dbDNP and db_xref annotations now hyperlinked.
Greater file format capability
More options for VCF, SWF, FASTQ, SAM/BAM and zipped files
New ‘info’ tab
View lineage, history and edit common fields in properties such as description and organism
R6 Update
7 February 2013
6.1 release notes
No more binding to known repeats
The Primer Design tools now include a repeat library mispriming function that prevents binding to known repeats, saving you time.
Custom BLAST now supports BLAST+ for increased search performance.
Enhanced DNA/RNA fold view
The DNA/RNA fold view is now available on two sequences, showing how the two molecules bind to each other (using Vienna rnacofold 2.0.7). We’ve also updated the stats panel, improved the probability color scheme, and implemented new energy models, giving you greater visibility than ever.