Affordable and comprehensive primer design and testing

Don’t pay for primer design software separately or fiddle with online tools. Geneious does it better. Save on ordering new primers and get better yield without the tedium of repeated wet laboratory experiments.

Built in primer database and browser

Drag and drop your primers in FASTA, spreadsheet or Genbank format. Once loaded, you'll have them all as primers in your own searchable database. Geneious will let you know if you already have primers that match a new sequence.

Elegant primer design

Design primers and hybridization probes to any target region or entire sequence directly onto alignments and assemblies in the beautiful Geneious sequence viewer. Add and remove extensions to a primer sequence before, during or after the design process.

Test primers against any sequence before your order

Geneious is packed full of features to help you get the most from your PCR experiments.

  • Screen for physical properties, hairpins and primer-dimers.
  • Select all PCR products on a sequence or any two primers in a pair to extract the product of those primers.
  • 5' primer extension – Extend a primer with a restriction site, polyA tail or any other custom sequence
  • Dynamically calculate the DNA melting point for a selected region for manual primer design


Download a free trial and one of our tutorials to get to grips with the functionality of Geneious interactively.

Tutorial- Primer Design

Learn how to design basic and degenerate PCR primers, add 5 extensions and test primers in your database.

Tutorial- Topo Cloning

Learn how to simulate Topo cloning in Geneious, using TA, Blunt or Directional methods.