Primer Design and Testing

Drag and drop your primers in FASTA, spreadsheet or Genbank format. Once loaded, you’ll have them all as primers in your own searchable database. Geneious Prime will let you know if you already have primers that match a new sequence.

Design PCR and sequencing primers and hybridization probes, to any target region or entire sequence, directly on alignments and assemblies in the Geneious sequence viewer. Add and remove extensions to a primer sequence before, during or after the design process.

Be confident your primers will only bind in a single location with automatic identification of any additional, off target, binding sites found when using Test with Saved Primers. Off-target information for the tested document will be added to the new primer annotations.

Geneious Prime is packed full of features to help you get the most from your PCR experiments.

• Screen for physical properties, hairpins and primer-dimers
• Select all PCR products on a sequence or any two primers in a pair to extract the product of those primers
• 5′ primer extension – Extend a primer with a restriction site, polyA tail or any other custom sequence
• Dynamically calculate the DNA melting point for a selected region for manual primer design