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Master Geneious and key concepts in molecular biology and bioinformatics. FREE unlimited access to online courses.
Getting Started
VIDEO SERIES

VIDEO SERIESA Brief Tour of Geneious Prime
Get up and running quickly with this brief tour of the Geneious Prime interface.

VIDEO SERIESGet Started with Geneious Prime
Get started with your molecular biology and sequence analysis in Geneious Prime, from importing your data to aligning your first sequences.

VIDEO SERIESGet Started with Geneious Biologics
Get started with your antibody analysis in Geneious Biologics, from activating your account to understanding your results and aligning your first sequences.
Next Generation Sequencing
WRITTEN TUTORIALS

TUTORIALHow to Run De Novo Assembly in Geneious Prime
Assemble paired Illumina MiSeq reads into contigs without a reference, then check the result against the E. coli genome.

TUTORIALHow to Map NGS Reads to a Reference and Call Variants
Map paired reads to the E. coli yghJ CDS, review coverage, then call and filter SNPs on the assembly.

TUTORIALHow to Analyze a Metagenomic Dataset in Geneious Prime
Assemble, filter and classify a 16S rRNA V4 amplicon dataset from paired Illumina MiSeq reads.

TUTORIALHow to Measure Gene Expression in Geneious Prime
Measure RPKM, FPKM and TPM on RNA-Seq data from two sample conditions, then calculate differential expression.

TUTORIALHow to Run Expression Analysis with DESeq2 in Geneious Prime
Use DESeq2 to find which Vibrio fischeri genes change expression during colonization of the bobtail squid.

TUTORIALHow to use the Sequence Classifier in Geneious Prime
Classify mitochondrial sequences from subfossil bones and identify which kiwi species they came from.
Sanger Sequencing and Microsatellites
WRITTEN TUTORIALS

TUTORIALHow to Assemble Chromatograms in Geneious Prime
Assemble bidirectional Sanger reads from blue tit mitochondrial DNA and extract high-quality consensus sequences.

TUTORIALHow to Analyze Microsatellite Traces in Geneious Prime
Fit a ladder, call peaks, bin alleles and produce a genotype table with the Microsatellite plugin.
Learn Sequence Analysis
GUIDES

GUIDEDNA Sequencing Methods: Sanger, NGS and Long-Read
Learn the basics of DNA sequencing with this introductory article on Sanger, Next Generation and Long-read methods.

GUIDESequence Assembly: De Novo vs Mapping to a Reference
Learn the basics with this introductory guide to assembling DNA sequences with de novo and map to reference.

GUIDESequence Alignment Methods: Pairwise vs Multiple
Learn the basics of sequence alignment with this overview on the different methods used to align sequences.

GUIDEPhylogenetic Analysis: Tree-Building Methods Compared
Learn the basics of phylogenetics with this overview of phylogenetic trees how they work and how to build them.

GUIDEMolecular Cloning Techniques Compared: How to Choose
Learn the basics in Molecular Cloning with this overview article that covers restriction enzyme cloning to golden gate cloning.
Alignments and Tree Building
WRITTEN TUTORIALS

TUTORIALHow to Build Phylogenetic Trees in Geneious Prime
Build a phylogenetic tree from HIV and SIV sequences to trace which African primates HIV came from.

TUTORIALHow to Compare Pairwise Alignment in Geneious Prime
Compare Salmonella and E. coli genomes with dot plots, then align a pygmy chimp sequence against a mutated copy.

TUTORIALHow to Align Genomes with Mauve in Geneious Prime
Align three M. tuberculosis complex genomes with Mauve, then order a draft assembly against a reference.

TUTORIALHow to Run Molecular Analysis of Plant DNA in Geneious Prime
Annotate plant DNA sequences with BLAST and multiple alignment, then read a tree to compare genetic distance.
Annotate and Visualize
WRITTEN TUTORIALS

TUTORIALHow to Transfer Annotations by Homology in Geneious Prime
Transfer features to a reference or consensus sequence in an alignment or assembly.

TUTORIALHow to Transfer Annotations by Alignment in Geneious Prime
Transfer annotations between sequences within an alignment using the Copy To function.

TUTORIALHow to Transfer Annotations From Database in Geneious Prime
Transfer homologous annotations from a custom feature database with the Annotate From tool."

TUTORIALHow to Visualize 3D Protein Structures in Geneious Prime
Explore the controls in the 3D structure viewer and visualize active sites in a protein structure.
Molecular Cloning
WRITTEN TUTORIALS

TUTORIALHow to Simulate Restriction Cloning in Geneious Prime
Ligate a PCR product into pET26b, in frame with PelB for periplasmic export and a C-terminal His-tag.

TUTORIALHow to Simulate Gibson Assembly in Geneious Prime
Assemble a human DCN-eGFP fusion gene by Gibson Assembly, then batch clone it with different promoters.

TUTORIALHow to Simulate Golden Gate Cloning in Geneious Prime
Assemble six parts into a complete GFP coding sequence using BsaI overhangs and the pGoldenGate-SE7 backbone.

TUTORIALHow to Perform Cloning Validation in Geneious Prime
Group reads into clones, map them to a reference, and automate validation with the Cloning Validation tool.

TUTORIALHow to Simulate Single Site Gateway® Cloning in Geneious Prime
Design attB-linked primers for the mature xynB CDS, then simulate the BP and LR reactions into pDest17.

TUTORIALHow to Simulate Multisite Gateway® Cloning in Geneious Prime
Join Drosophila trpA1 regulatory elements to a yeast GAL4 activator using multisite Gateway cloning.

TUTORIALHow to Simulate TOPO™ Cloning in Geneious Prime
Clone human proinsulin into TOPO vectors using TA, Blunt and Directional methods, and compare the three.
Primer Design and CRISPR
WRITTEN TUTORIALS

TUTORIALHow to Design PCR Primers in Geneious Prime
Import, design and test oligonucleotide primers, primer pairs, and primer-probe combinations.

TUTORIALHow to Analyze CRISPR Editing in Geneious Prime
Prepare sequence files, set up a CRISPR editing analysis, and interpret the results.

TUTORIALHow to Find CRISPR Sites in Geneious Prime
Find guide RNA sites in the LYP1 gene of baker's yeast, then screen the whole genome for off-target binding.
BLAST, NCBI & GenBank
WRITTEN TUTORIALS

TUTORIALHow to Run BLAST Searching in Geneious Prime
BLAST a sequence against GenBank or a custom database, run batch searches, and export the hits.

TUTORIALHow to Access GenBank from Geneious Prime
Search GenBank from inside Geneious, download nucleotide sequences, taxonomy and publications, and run BLAST.

TUTORIALHow to Submit to GenBank from Geneious Prime
Format sequences and alignments for GenBank, add the required metadata, and correct annotation qualifiers.
Automation & Customization
WRITTEN TUTORIALS

TUTORIALHow to Build Automated Workflows in Geneious Prime
Build a workflow that runs from raw shark barcoding chromatograms to a finished phylogenetic tree in one pass.
Antibody Discovery
VIDEO SERIES

VIDEO SERIESGet Started with Geneious Biologics
Get started with your antibody analysis in Geneious Biologics, from activating your account to understanding your results and aligning your first sequences.

VIDEO SERIESAntibody Discovery with Geneious Biologics
Deepen your understanding of key concepts in antibody discovery and master the broad range of Geneious Biologics tools with this in-depth video series.
Get started with Geneious today