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Getting Started

Get up and running quickly with this brief tour of the Geneious Prime interface.
Get started with your molecular biology and sequence analysis in Geneious Prime, from importing your data to aligning your first sequences.
Get started with your antibody analysis in Geneious Biologics, from activating your account to understanding your results and aligning your first sequences.

Next Generation Sequencing

Assemble paired Illumina MiSeq reads into contigs without a reference, then check the result against the E. coli genome.
Map paired reads to the E. coli yghJ CDS, review coverage, then call and filter SNPs on the assembly.
Assemble, filter and classify a 16S rRNA V4 amplicon dataset from paired Illumina MiSeq reads.
Measure RPKM, FPKM and TPM on RNA-Seq data from two sample conditions, then calculate differential expression.
Use DESeq2 to find which Vibrio fischeri genes change expression during colonization of the bobtail squid.
Classify mitochondrial sequences from subfossil bones and identify which kiwi species they came from.

Sanger Sequencing and Microsatellites

Assemble bidirectional Sanger reads from blue tit mitochondrial DNA and extract high-quality consensus sequences.
Fit a ladder, call peaks, bin alleles and produce a genotype table with the Microsatellite plugin.

Learn Sequence Analysis

Learn the basics of DNA sequencing with this introductory article on Sanger, Next Generation and Long-read methods.
Learn the basics with this introductory guide to assembling DNA sequences with de novo and map to reference.
Learn the basics of sequence alignment with this overview on the different methods used to align sequences.
Learn the basics of phylogenetics with this overview of phylogenetic trees how they work and how to build them.
Learn the basics in Molecular Cloning with this overview article that covers restriction enzyme cloning to golden gate cloning.

Alignments and Tree Building

Build a phylogenetic tree from HIV and SIV sequences to trace which African primates HIV came from.
Compare Salmonella and E. coli genomes with dot plots, then align a pygmy chimp sequence against a mutated copy.
Align three M. tuberculosis complex genomes with Mauve, then order a draft assembly against a reference.
Annotate plant DNA sequences with BLAST and multiple alignment, then read a tree to compare genetic distance.

Annotate and Visualize

Transfer features to a reference or consensus sequence in an alignment or assembly.
Transfer annotations between sequences within an alignment using the Copy To function.
Transfer homologous annotations from a custom feature database with the Annotate From tool."
Explore the controls in the 3D structure viewer and visualize active sites in a protein structure.

Molecular Cloning

Ligate a PCR product into pET26b, in frame with PelB for periplasmic export and a C-terminal His-tag.
Assemble a human DCN-eGFP fusion gene by Gibson Assembly, then batch clone it with different promoters.
Assemble six parts into a complete GFP coding sequence using BsaI overhangs and the pGoldenGate-SE7 backbone.
Group reads into clones, map them to a reference, and automate validation with the Cloning Validation tool.
Design attB-linked primers for the mature xynB CDS, then simulate the BP and LR reactions into pDest17.
Join Drosophila trpA1 regulatory elements to a yeast GAL4 activator using multisite Gateway cloning.
Clone human proinsulin into TOPO vectors using TA, Blunt and Directional methods, and compare the three.

Primer Design and CRISPR

Import, design and test oligonucleotide primers, primer pairs, and primer-probe combinations.
Prepare sequence files, set up a CRISPR editing analysis, and interpret the results.
Find guide RNA sites in the LYP1 gene of baker's yeast, then screen the whole genome for off-target binding.

BLAST, NCBI & GenBank

BLAST a sequence against GenBank or a custom database, run batch searches, and export the hits.
Search GenBank from inside Geneious, download nucleotide sequences, taxonomy and publications, and run BLAST.
Format sequences and alignments for GenBank, add the required metadata, and correct annotation qualifiers.

Automation & Customization

Build a workflow that runs from raw shark barcoding chromatograms to a finished phylogenetic tree in one pass.

Antibody Discovery

Get started with your antibody analysis in Geneious Biologics, from activating your account to understanding your results and aligning your first sequences.
Deepen your understanding of key concepts in antibody discovery and master the broad range of Geneious Biologics tools with this in-depth video series.
Get started with Geneious today