Geneious Updates and Downloads

Geneious Prime 2027.0

• Enhanced CRISPR off-target analysisA new Off-Target viewer surfaces the 100 highest-scoring off-target sites for a guide of interest, so you can investigate each in detail. The CFD off-target score (Doench et al 2016) is now available, and you can run multiple on-target and off-target scoring algorithms in a single operation. Off-target searching across genomes is also up to three times faster, and you can filter results by setting a threshold for each score, returning only guides that meet it.
• More flexible cloningCloning gives you more control over your backbone and inserts. In Gibson and In-Fusion cloning you can choose any backbone fragment from the tag dropdown when a circular backbone is digested into multiple pieces. Golden Gate cloning now supports adding spacers, and changes to your input documents can be propagated through to a Parts cloning result.
• Align circular sequencesYou can now align circular sequences. This is specifically for aligning circular sequences: Geneious treats them as circular rather than linear, so you no longer need to linearize them first. Alignments of linear sequences are unaffected.

New feature highlights for Geneious Biologics include Analysis Workflows for batching NGS Antibody Annotation, a Most Common Representative Sequence option, interactive graphs with colorblind-friendly palettes, and more.

• Analysis Workflows for batching NGS Antibody Annotation
• New Representative Sequence option: Most Common
• Graphing and plots:
  • Interactive graphs, plus colorblind-friendly palettes
  • Color clusters in Networks and Trees by labels
• New Reference Databases: Mouse TCR and Rabbit IgG
  

Geneious Prime 2026.1

• One-step multiple insertion point Parts Cloning (Enterprise only)Quickly build constructs with non-contiguous fragments inserted at multiple locations within your backbone vector in a single step. Circular backbones are now accepted and can be linearized by selecting a cut point, or by excising an annotation or interval to create the insertion site.
• Run multiple CRISPR algorithms at onceThe Find CRISPR Sites operation now runs multiple algorithms simultaneously from the Cloud Workspace, saving time when evaluating guide RNA candidates across different scoring methods. Two new scoring algorithms have been added: the Cutting Frequency Determination off-target score published by Doench et al. (2016), and a new on-target scoring algorithm published by Doench et al. (2022) that accounts for tracrRNA variants.
  
  
  

Geneious Prime 2026.0

• Pin Favorite folders for easy accessAdd folders from your Cloud and Local databases to the new favourites  section, for quick and easy access to your most commonly used files.  Favourites are remembered across devices when connected to the cloud workspace.
• SnapGene History ImportImport of SnapGene *.dna files now creates Fragment annotations for the first-level History, showing a record of the simulation method for certain cloning types, and enabling resurrection of parent files directly within Geneious Prime.
  
  

New feature highlights for Geneious Biologics include HCAb Protein Statistics, Custom Scatterplots, Deleted Items folder and more.

• Protein Statistics extended to HCAb molecules
• Updated Alpaca Ig 2025 reference database
• Deleted Items folder
• New Visualization: Custom Plots for assay data etc.
• Improved NGS Antibody Annotation speed
• More filtering options for NGS Annotations

Geneious Prime 2025.2

• Create and save custom Primer Extensions: Choose sequences from your Geneious database to use as primer extensions when designing primers, with annotations carried through to your PCR products.
• Advanced Cloud Workflow sharing: Save workflows in the Cloud, and share them with colleagues. You can now share Cloud Workflows with specific people or groups within your organisation, in read-only mode.
  

Geneious Prime 2025.1

• Apple Silicon: Speed up your analyses by up to 40% or more on macOS with Geneious Prime now running natively on Apple Silicon (ARM64 architecture).
• Cloning Validation Enhancements:Easily group and batch align your sequencing data to virtual clones with usability and performance improvements to the cloning validation tool.
• Cloud Workspace: Backup your data and streamline collaboration in the cloud. This release includes performance improvements, support for restoring Deleted Items, and extended sharing options.

New feature highlights for Geneious Biologics include mAb and fAb Protein Statistics, NGS/Barcode/UMI support for Peptide Annotator and more.

• Protein Statistics calculated for whole molecules - mAb and fAb
• NGS, Barcode and UMI functionality for Peptide Annotator
• Updated Human and Mouse Ig Reference databases
• Streamlined Graph Selection

Geneious Prime 2025.0

• QC Report for NGS Sequences: Quickly visualise overall NGS data quality in the new QC Report tab, calculated using the FastQC tool from Babraham Bioinformatics.
• Geneious Cloud: Backup your data and streamline collaboration with significant performance enhancements and improved sharing options for Geneious Cloud.

New feature highlights for Geneious Biologics include cluster relationships visualization, assay data heat maps for circular trees and other improvements.

• Cluster Relationships Visualization
• Cluster Similarity Network and Tree Visualizations
• Assay Data Heatmaps for Circular Trees
• Extended Alignment Flexibility and Sequence Export from Alignments
• Other Improvements

New feature highlights for Geneious Biologics include support for non-antibody therapeutic peptides and the ability to share your analysis options with your team.

• Analysis of non-antibody therapeutic peptides and proteins
• Easily accessible new Graphs tab
• Save and share analysis settings within your organization
• Better support for scFv/bi-specifics: Linker databases

Geneious Prime 2024.0 Feature Overview

Watch for an overview of the new features in Geneious Prime 2024.0

• Cloning Validation: New visual and semi-automated step to add, export and record validation metadata during batch plasmid validation.
• Map to Reference Algorithm Advisor: Interactive quiz to help you decide which algorithm is the best fit for you data.

Exciting new features for Geneious Biologics include manual sequence editing, dual nanobody (VHH-VHH) support, and protein scaffold reference databases.

• Manual Sequence Editing
• NGS Antibody Annotator
• Enrichment and Alignment Visualizations
• VHH and Dual Nanobody Analysis
• Reference Database Creator

Geneious Prime 2023.2 Feature Overview

Watch for an overview of the new features in Geneious Prime 2023.2

• De Novo Assembly Algorithm Advisor: Interactive quiz to help you decide which algorithm is the best fit for your data.
• Cloud Personal Workspace: Access your data from multiple computers with secure personal data storage in Geneious Prime.
• Export Lengths Graph Data: Quickly and easily export a csv file with your sequence length distribution data.
• Improvements to STAR RNA-seq aligner: You can now use the STAR RNA-seq aligner in Geneious with multiple references.

Exciting new features for Geneious Biologics include:

• An improved user interface experience
• Positional Liabilities based on antibody numbering schemes
• Adding labels to a selection of sequences
• Clustering options now available in Single Clone Antibody Analysis
• New speedier integration with Geneious Prime

New Features in Geneious Prime 2023.1

• Dotmatics Platform Integration plugin: Launch the registration workflow to register your sequences in Dotmatics Bioregister.
• Import .sbd files: Geneious Prime can now import DNAStar Lasergene SeqBuilder(Pro) .sbd file format.
• Geneious Cloud Database: Access your data from multiple computers with secure personal data storage in Geneious Prime.

New features for Geneious Biologics include:

• More Numbering Schemes for annotation
• Streamlined Extract/Export Features
• Extract & Recluster
• Import Sequences from .csv and excel files

Geneious Prime 2023.0 Feature Overview

Watch for an overview of the new features in Geneious Prime 2023.0

• Primer Specificity Testing: Design target-specific primers in one step with new primer specificity testing.
• Cloning Validation: New visual tool for automating sequence alignment during batch plasmid validation
• STAR RNA-seq mapper: Added STAR, a RNA-seq mapper that performs highly accurate spliced sequence alignment.
• CRISPR Improvements: Added more PAMs and the ability to select individual sequences for specificity testing.

What's New in Geneious Prime 2022.2?

• In-Fusion cloning: Perform low or high throughput In-Fusion Cloning with pre-optimized settings.
• Parts Cloning Support for Proteins: Scale up your construct engineering with combinatorial assembly of proteins.
• Primer Extension Detection: Automatically detect and annotate extensions on your primer databases during import.

New features and functionality for Geneious Biologics includes:

• Customizable clustering options for Antibody Annotator
• History Viewer tab to track input data and more across documents
• Copy-paste sequences for Quick Analysis
• Chain Combinations tables in Single Clone Antibody Analysis