Geneious Prime

Molecular Cloning

Automatically annotate plasmid maps and expression vectors. Simulate a variety of molecular cloning operations including restriction cloning, Gibson Assembly, Gateway cloning and TOPO cloning.

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Simulate molecular cloning operations

Ligate multiple fragments together that have matching overhangs
Insert a fragment of DNA into a vector with matching overhangs
Simulate:
- Restriction cloning and PCR
- Golden Gate cloning
- In-Fusion Cloning and Gibson Assembly
- Gateway and TOPO cloning
- Parts cloning

Automatically annotate plasmid maps and expression vectors

Geneious Prime automatically highlights plasmids with common promoters, terminators, cloning sites, restriction sites, reporter genes, affinity tags, selectable marker genes, replication origins and open reading frames.

Codon Optimization Tool

Find rare codons or produce a fully optimized sequence based on a target organism and remove unwanted restriction sites at the same time.

Store and access important restriction enzyme and DNA fragment data

Drag and drop your important data, customize your restriction enzymes lists and display all of the enzymes on your linear or circular DNA fragment-all in real time. Commercially available enzymes including GoldenGate and BioBrick are already stored in Geneious Prime.