Restriction Cloning

Find and analyze restriction enzymes and simulate restriction cloning of a single insert into a vector backbone.

Course Content

Find and Analyze Restriction Enzymes

INSIDE THE VIDEO
Select enzymes to simulate an agarose gel, view and export fragment information, and create a custom enzyme set.
LENGTH
5 minutes

Perform Restriction Cloning

INSIDE THE VIDEO
Select a vector and insert for restriction cloning, choose enzymes, add additional inserts or rearrange inserts, and view the final cloning product.
LENGTH
3 minutes

Recommended Resources

How do I create a custom enzyme set?

Learn how to create a specific set of enzymes that you want to use repeatedly.

How do I find non-cutting restriction enzymes?

Select an enzyme set and produce a new document table of all non-cutters.

Can Geneious Prime find nicking enzyme (endonuclease) sites?

The "Find restriction sites" tool can be used to find and annotate sites for nicking endonucleases.

Manual for Restriction Cloning

A guide for using the restriction cloning tools in Geneious.

More Geneious Academy

Practice simulating restriction cloning. Ligate PCR product into an expression vector and confirm CDS in-frame fusion.
Watch the series to learn the basics of Golden Gate cloning and how to simulate standard or batch Golden Gate cloning.
Watch this 3 part series on simulating TOPO cloning in Geneious Prime using either the TA, blunt or directional method.
In this series learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.
Get started with Geneious today