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Course Content
Find and Analyze Restriction Enzymes
INSIDE THE VIDEO
Select enzymes to simulate an agarose gel, view and export fragment information, and create a custom enzyme set.
LENGTH
5 minutes
Perform Restriction Cloning
INSIDE THE VIDEO
Select a vector and insert for restriction cloning, choose enzymes, add additional inserts or rearrange inserts, and view the final cloning product.
LENGTH
3 minutes
Recommended Resources
How do I create a custom enzyme set?
Learn how to create a specific set of enzymes that you want to use repeatedly.
How do I find non-cutting restriction enzymes?
Select an enzyme set and produce a new document table of all non-cutters.
Can Geneious Prime find nicking enzyme (endonuclease) sites?
The "Find restriction sites" tool can be used to find and annotate sites for nicking endonucleases.
Manual for Restriction Cloning
A guide for using the restriction cloning tools in Geneious.
More Geneious Academy

TUTORIALPractice Restriction Cloning
Practice simulating restriction cloning. Ligate PCR product into an expression vector and confirm CDS in-frame fusion.

VIDEO SERIESGolden Gate Cloning
Watch the series to learn the basics of Golden Gate cloning and how to simulate standard or batch Golden Gate cloning.

VIDEO SERIESTOPO Cloning
Watch this 3 part series on simulating TOPO cloning in Geneious Prime using either the TA, blunt or directional method.

VIDEO SERIESPrimer Design
In this series learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.
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