Primer Design

Learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.

INSIDE THE VIDEO

Automatically design new primers, adjust melting temperature, length, hairpin and GC content, and extract PCR product from a primer pair.

LENGTH

6 minutes

INSIDE THE VIDEO

Learn to import primer sequences to Geneious Prime using a delimited text file or from a spreadsheet by copying and pasting.

LENGTH

3 minutes

INSIDE THE VIDEO

Learn the two methods for testing primers against a target sequence: add primers to a sequence or test with saved primers.

LENGTH

3 minutes

INSIDE THE VIDEO

Learn three ways to add 5-prime extensions, manually, with the design new primers tool or by editing existing primer sequences.

LENGTH

4 minutes

Recommended Resources

Common questions on using primers in Geneious.

Target specific primers and test their specificity against an off-target sequence(s).

Primers are always 5’ to 3’ so in Geneious if you reverse complement a primer.

A guide to using the primer tools in Geneious.

Practice how to import, design and test oligonucleotide primers, primer-pairs and primer-pair/probe combinations.

In this series, learn to find, annotate and check CRISPR sites, prepare sequence files, analyze, and interpret results.

Watch this video series to learn the basics of Gateway cloning and how to simulate single or multisite Gateway cloning.

Learn how to search with BLAST. Single or batch BLAST your sequence against GenBank or custom databases to find similar sequences.

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