Primer Design in Geneious Prime

Course Content

How to Design Primers

Automatically design new primers, adjust melting temperature, length, hairpin and GC content, and extract PCR product from a primer pair.
LENGTH 6 minutes

Import Primers

Learn to import primer sequences to Geneious Prime using a delimited text file or from a spreadsheet by copying and pasting.
LENGTH 3 minutes

Test with Saved Primers

Learn the two methods for testing primers against a target sequence: add primers to a sequence or test with saved primers.
LENGTH 3 minutes

Design Primers with 5′ Extensions

Learn three ways to add 5-prime extensions, manually, with the design new primers tool or by editing existing primer sequences.
LENGTH 4 minutes

Related Geneious Articles

Testing Primers Against a Whole Genome

Target specific primers and test their specificity against an off-target sequence(s).

Designing Primers for Gibson Assembly

Learn more in the Gibson Assembly tutorial

How to Extract Multiple Primers at Once

You can use the Tools → Extract Annotations operation to extract primers

Probe Design for qPCR and Taqman applications

How to create primer/probe sets suitable for Taqman or quantitative PCR (qPCR) assays.

More Geneious Academy

TUTORIALHow to Design PCR Primers in Geneious Prime
Import, design and test oligonucleotide primers, primer pairs, and primer-probe combinations.
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Find, annotate and check CRISPR sites, prepare sequence files, analyze, and interpret results.
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Simulate single or multisite Gateway® cloning, perform BP and LR reactions, design primers to amplify your sequence and incorporate attB sites.
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VIDEO SERIESSearching with BLAST in Geneious Prime
Search with BLAST against GenBank or a custom database, running single or batch queries to find similar sequences.
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