- Products
- Enterprise
- Resources
- Support
- Pricing
Course Content
How to Design Primers
INSIDE THE VIDEO
Automatically design new primers, adjust melting temperature, length, hairpin and GC content, and extract PCR product from a primer pair.
LENGTH
6 minutes
Import Primers
INSIDE THE VIDEO
Learn to import primer sequences to Geneious Prime using a delimited text file or from a spreadsheet by copying and pasting.
LENGTH
3 minutes
Test with Saved Primers
INSIDE THE VIDEO
Learn the two methods for testing primers against a target sequence: add primers to a sequence or test with saved primers.
LENGTH
3 minutes
Design Primers with 5′ Extensions
INSIDE THE VIDEO
Learn three ways to add 5-prime extensions, manually, with the design new primers tool or by editing existing primer sequences.
LENGTH
4 minutes
Related Geneious Articles
Testing Primers Against a Whole Genome
Target specific primers and test their specificity against an off-target sequence(s).
Designing Primers for Gibson Assembly
Learn more in the Gibson Assembly tutorial
How to Extract Multiple Primers at Once
You can use the Tools → Extract Annotations operation to extract primers
Probe Design for qPCR and Taqman applications
How to create primer/probe sets suitable for Taqman or quantitative PCR (qPCR) assays.
More Geneious Academy

TUTORIALHow to Design PCR Primers in Geneious Prime
Practice how to import, design and test oligonucleotide primers, primer-pairs and primer-pair/probe combinations.

VIDEO SERIESWorking with CRISPR
In this series, learn to find, annotate and check CRISPR sites, prepare sequence files, analyze, and interpret results.

VIDEO SERIESGateway Cloning
Watch this video series to learn the basics of Gateway cloning and how to simulate single or multisite Gateway cloning.

VIDEO SERIESSearching with BLAST
Learn how to search with BLAST. Single or batch BLAST your sequence against GenBank or custom databases to find similar sequences.
Get started with Geneious today