Searching with BLAST in Geneious Prime

This BLAST searching series shows how to run a single BLAST search in Geneious Prime against GenBank or a custom database, reviewing hits by E value, identity, and length in a hit table or query-centric view. A second video covers batch BLASTing a sequence list with an NCBI API key, filtering results into has-hit and no-hit lists. Two videos, 4 to 5 minutes each.

Course Content

Introduction to BLAST Searching

Run a BLAST search against GenBank’s nucleotide collection or a custom database, viewing results as a hit table or query-centric alignment. Review E value, percentage identity, and hit length, then download the full sequence for annotations.
LENGTH 5 minutes

Batch BLAST Searching

Add an NCBI API key in Preferences for faster batch BLAST searches, then BLAST a sequence list using Megablast (switch to blastn for more sensitivity). Filter results by has-hit or no-hit, useful for screening out non-target contamination.
LENGTH 4 minutes

Recommended Resources

NCBI and BLAST

Common questions on how to use NCBI and BLAST in the Geneious interface.

Searching and BLAST

Direct access to NCBI public BLAST databases, custom BLAST and integrated GenBank search.

Setting up shared BLAST for multiple users

The easiest ways to set up shared BLAST databases for multiple users

Manual for BLAST

A guide to using the BLAST tool in Geneious.

More Geneious Academy

TUTORIALHow to Run BLAST Searching in Geneious Prime
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