Working with CRISPR in Geneious Prime

Course Content

Finding CRISPR Sites

Use the CRISPR tool in Geneious Prime to find and annotate CRISPR sites in a sequence and check for off-target binding sites.
LENGTH 5 minutes

Finding Paired CRISPR Sites

Use the CRISPR tool in Geneious Prime to find paired sites in a sequence.
LENGTH 3 minutes

Merging Paired Reads

Learn how to prepare your data for CRISPR analysis by merging paired reads.
LENGTH 2 minutes

Analyze CRISPR Editing Results

Analyze CRISPR editing results in Geneious Prime, including how to prepare your sequence files, set up the analysis, and interpret the results.
LENGTH 3 minutes

Recommended Resources

How do I set the off-target database for CRISPR?

Learn to search for off-target binding sites against a database of sequences.

Manual for CRISPR site finder

The Find CRISPR Sites tool searches for gRNA sites in your selected sequences.

Manual for CRISPR

A guide to using the CRISPR tools in Geneious Prime.

More Geneious Academy

TUTORIALHow to Find CRISPR Sites in Geneious Prime
Find guide RNA sites in the LYP1 gene of baker's yeast, then screen the whole genome for off-target binding.
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TUTORIALHow to Analyze CRISPR Editing in Geneious Prime
Prepare sequence files, set up a CRISPR editing analysis, and interpret the results.
Read Tutorial
VIDEO SERIESAnnotate Sequences in Geneious Prime
Annotate your sequences by customizing the view, adding new annotations or annotating your sequence with BLAST.
Watch Series
VIDEO SERIESPrimer Design in Geneious Prime
View, design and export primers for many applications such as PCR, cloning or sequencing.
Watch Series
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