Working with CRISPR

How to find, annotate and check CRISPR sites, prepare sequence files, analyze, and interpret the results.

Course Content

Finding CRISPR Sites

INSIDE THE VIDEO
Use the CRISPR tool in Geneious Prime to find and annotate CRISPR sites in a sequence and check for off-target binding sites.
LENGTH
5 minutes

Finding Paired CRISPR Sites

INSIDE THE VIDEO
Use the CRISPR tool in Geneious Prime to find paired sites in a sequence.
LENGTH
3 minutes

Merging Paired Reads

INSIDE THE VIDEO
Learn how to prepare your data for CRISPR analysis by merging paired reads.
LENGTH
2 minutes

Analyze CRISPR Editing Results

INSIDE THE VIDEO
Analyze CRISPR editing results in Geneious Prime, including how to prepare your sequence files, set up the analysis, and interpret the results.
LENGTH
3 minutes

Recommended Resources

How do I set the off-target database for CRISPR?

Learn to search for off-target binding sites against a database of sequences.

Manual for CRISPR site finder

The Find CRISPR Sites tool searches for gRNA sites in your selected sequences.

Manual for CRISPR

A guide to using the CRISPR tools in Geneious Prime.

More Geneious Academy

Practice finding and annotating CRISPR sites in a sequence. Check against a genome for off-target binding sites.
Practice analyzing CRISPR editing results, preparing sequences, setting up the analysis and interpreting results.
Learn to annotate your sequences by customizing the view, adding new annotations or annotating your sequence with BLAST.
In this series learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.
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