AI-powered end-to-end solution for antibody discovery—from molecular modeling to wet lab, data analysis, and registration.
Geneious Prime supports both single-site and multisite Gateway cloning. The software simulates BP reactions (generating an entry clone from an attB PCR product and a pDONR™ vector), LR reactions (transferring inserts into destination vectors), and combined single-step BP+LR reactions. Multisite Gateway workflows with multiple attB sites are also supported for assembling multiple inserts in a single reaction.
Geneious Prime supports multisite Gateway cloning for assembling multiple inserts from separate entry clones into a single destination vector. The software guides selection of the appropriate attB site combinations for multisite reactions and simulates the final construct, confirming that all att site recombination events produce the intended arrangement of inserts.
Geneious Prime automatically designs Gateway cloning primers with the correct attB site extensions. The tool adds the required four guanine residues at the 5′ end, the 25 bp attB motif, appropriate spacers, and at least 18–25 bp of gene-specific sequence — including the requirement that attB1 sites end with thymidine. Researchers specify the gene-specific region; Geneious Prime handles the att site architecture.
Manually designing Gateway primers requires calculating attB site sequences, spacers, and Tm values across primer components — a process prone to sequence errors that only surface after PCR. Geneious Prime automates att site primer design and simulates the complete reaction, flagging potential issues before any reagents are ordered. The visual construct output also provides a clear record of the intended design for documentation.
Geneious Prime lets you simulate the complete Gateway cloning reaction in silico before committing to reagent orders. The software extracts and validates PCR products, simulates BP and LR reactions, and displays the annotated final construct — so you can confirm insert orientation, verify att site placement, and check the expression clone sequence before running the reaction in the lab.
Gateway cloning simulation is available in all Geneious Prime subscription plans, including Student, Academic, Corporate personal tiers, as well as Team and Enterprise plans. A free trial is available with no commitment required to evaluate the Gateway cloning workflow before purchasing.
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