Gateway Cloning

Learn how Gateway cloning works and how to clone single or multiple fragments into an expression clone.

Course Content

Gateway Cloning with a Single Insert

Perform BP and LR reactions, design primers to amplify your sequence, incorporate AttB sites, and simulate a one-step Gateway reaction.
9 minutes

Gateway Cloning with Multiple Inserts

Understand the concepts behind multisite Gateway cloning, choose attB sites for correct insertion, design primers, and simulate BP and LR reactions.
7 minutes

Recommended Resources

How to add primers to Geneious Prime?

Add existing primers to Geneious Prime, either manually or by importing from external sources.

How do I design cloning primers?

Design primers to be exactly at the ends of the CDS.

Manual for Gateway Cloning

A guide to using the Gateway cloning tool in Geneious.

More Geneious Academy

Practice simulating gateway cloning. Automatically design attB-linked primers, and the BP and LR reactions.
Practice simulating multisite Gateway cloning. Choose attB sites, design primers, and simulate BP and LR reactions.
In this series learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.
Learn to annotate your sequences by customizing the view, adding new annotations or annotating your sequence with BLAST.
Get started with Geneious today