Gateway Cloning

Learn how Gateway cloning works and how to clone single or multiple fragments into an expression clone.

INSIDE THE VIDEO

Perform BP and LR reactions, design primers to amplify your sequence, incorporate AttB sites, and simulate a one-step Gateway reaction.

LENGTH

9 minutes

INSIDE THE VIDEO

Understand the concepts behind multisite Gateway cloning, choose attB sites for correct insertion, design primers, and simulate BP and LR reactions.

LENGTH

7 minutes

Recommended Resources

Add existing primers to Geneious Prime, either manually or by importing from external sources.

Design primers to be exactly at the ends of the CDS.

A guide to using the Gateway cloning tool in Geneious.

Practice simulating gateway cloning. Automatically design attB-linked primers, and the BP and LR reactions.

Practice simulating multisite Gateway cloning. Choose attB sites, design primers, and simulate BP and LR reactions.

In this series learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.

Learn to annotate your sequences by customizing the view, adding new annotations or annotating your sequence with BLAST.

Get started with Geneious today