Golden Gate Cloning

Learn the principles of Golden Gate cloning and how to simulate standard or batch Golden Gate cloning.

INSIDE THE VIDEO

Assess your DNA sequences, arrange your fragments and design primers, simulate Golden Gate cloning, and check your assembly.

LENGTH

6 minutes

INSIDE THE VIDEO

Perform cloning using a sequence list, rearrange parts, simulate Golden Gate cloning, and view your final product and primers.

LENGTH

2 minutes

Recommended Resources

Learn how to create a speciﬁc set of enzymes that you want to use repeatedly.

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A guide to using the Golden Gate cloning tool in Geneious.

Practice simulating Golden Gate cloning. Automatically design primers to generate overhangs for parts assembly.

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In this series learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.

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