Golden Gate Cloning

Learn the principles of Golden Gate cloning and how to simulate standard or batch Golden Gate cloning.

Course Content

Perform Golden Gate Cloning

Assess your DNA sequences, arrange your fragments and design primers, simulate Golden Gate cloning, and check your assembly.
6 minutes

Batch Golden Gate Cloning

Perform cloning using a sequence list, rearrange parts, simulate Golden Gate cloning, and view your final product and primers.
2 minutes

Recommended Resources

How do I create a custom enzyme set?

Learn how to create a specific set of enzymes that you want to use repeatedly.

How do I find non-cutting restriction enzymes?

Select an enzyme set and produce a new document table of all non-cutters.

Manual for Golden Gate Cloning

A guide to using the Golden Gate cloning tool in Geneious.

More Geneious Academy

Practice simulating Golden Gate cloning. Automatically design primers to generate overhangs for parts assembly.
Find and analyze restriction enzymes and simulate restriction cloning of a single insert into a vector backbone.
Learn how to simulate parts cloning and concatenate libraries of genetic elements outputting all possible combinations.
In this series learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.
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