Golden Gate cloning

Learn how to simulate Golden Gate cloning in Geneious, including how to automatically design oligonucleotide primers for generating the overhangs for assembly of parts. For Geneious R9 and above.

Download the Golden Gate cloning Tutorial

Tutorial Instructions

Geneious Education tutorials are installed by either 'Dragging and dropping' the zip file into Geneious or using File → Import → From File... in the Geneious menu. Do not unzip the tutorial.