Parts Cloning in Geneious Prime

This video shows how to simulate parts cloning in Geneious Prime, combining sequence lists or individual sequences, such as regulators, coding regions, and terminators, into a linearized vector or without a backbone. Reorder parts in the construct layout panel, optionally group results by a specified part, and review the full set of possible construct combinations.

Perform Parts Cloning

Arrange sequence lists or individual parts, such as regulators, coding regions, and terminators, into a linearized vector (or no backbone), then simulate parts cloning. Group results by a specific part, or combine everything into one sequence list.”

LENGTH 2 minutes

Common Questions about Part Cloning

What is Parts Cloning in Geneious Prime and when should I use it?

Parts Cloning in Geneious Prime is a combinatorial assembly workflow for researchers who need to arrange multiple genetic elements — such as promoters, coding sequences, and terminators — and generate all possible construct combinations from defined sequence libraries. It is suited for synthetic biology workflows, modular construct design, and any application requiring systematic exploration of part combinations before committing to bench assembly.

Can I validate the result of Parts Cloning assembly in Geneious Prime?

After running a Parts Cloning simulation, researchers can use Geneious Prime's Cloning Validation tool to confirm that the assembled constructs match their expected reference sequences. The recommended workflow moves directly from cloning simulation to validation, grouping Sanger reads into clones, mapping to the reference, and flagging any sequence variants before downstream experiments.

Can Geneious Prime generate all possible combinations from a parts library?

Geneious Prime's Parts Cloning tool generates all possible construct combinations from the sequence lists you define. Researchers organize their parts into positional groups, run the simulation, and Geneious Prime outputs the full combinatorial product set, allowing systematic review of every possible arrangement before selecting constructs for synthesis or bench assembly.

Is Parts Cloning available in all Geneious Prime subscription tiers?

Parts Cloning is available in all Geneious Prime subscription plans. The advanced one-step multiple insertion point variant is an Enterprise-tier feature requiring custom pricing. Standard Parts Cloning simulation (including combinatorial library generation) is included in Student, Academic, and Corporate personal plans, and in all Team plans.

How does Parts Cloning relate to the one-step multiple insertion point cloning feature?

Parts Cloning is the general multi-fragment combinatorial assembly workflow available across all Geneious Prime plans. One-step multiple insertion point Parts Cloning is an Academic and Enterprise feature that enables simultaneous insertion at multiple independent (non-adjacent) sites within a vector in a single cloning operation, using Gibson Assembly, In-Fusion or Golden Gate chemistry. Both workflows are accessible through the shared cloning interface.

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