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Sanger Sequence Analysis

Align your chromatograms to a reference sequence, find variants and verify sequences.

Intro to Sanger Sequencing Analysis

INSIDE THE VIDEO
Check sequence quality and trim the ends, map chromatogram sequences against a reference, and view the contig and edit bases.

  • Prepare your data
  • Align to a reference
  • View the contig

LENGTH
5 minutes

How To Find Variants with Sanger Sequencing

INSIDE THE VIDEO
Set parameters for calling SNPs, view SNPs information, see how variants affect the protein, and view and export polymorphisms.

LENGTH
2 minutes

Recommended Resources

SANGER SEQUENCING ANALYSIS

TUTORIAL
Practice Assembling Chromatograms

Practical exercise for the assembling chromatograms workflow.

FAQ
How do I assemble F and R pairs of sequences?

Use the Geneious de novo assembler to align pairs of Sanger sequences.

MANUAL
Creating, Viewing and Editing Sequences

Learn how to view, edit, translate, complement and translate sequences.

VIDEO SERIES
How To Design Primers

Learn how to view, design and export primers for PCR, cloning or sequencing.

GENEIOUS ACADEMY

How to perform de novo assembly
How to annotate sequences
How to perform restriction cloning
How to work with CRISPR