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Course Content
De Novo Assembly of NGS Reads
INSIDE THE VIDEO
Assemble paired-end data with the de novo assembler, compare full and normalized data sets, and view assembly results and consensus sequence.
LENGTH
5 minutes
Circular Contig Assembly
INSIDE THE VIDEO
Create circular contigs with the Geneious de novo assembler for a more accurate assembly of circular genomes.
LENGTH
1 minute
Recommended Resources
Plugin - BBDuk Trimmer
Download the plugin for quality trimming and filtering your sequences.
Plugin - MAFFT
Download the plugin for fast and accurate multiple sequence alignment with MAFFT
Geneious de novo assembly advisor decision tree & validation table
Use the decision tree to identify the most appropriate assembly algorithm for your data.
Manual for De Novo Assembly
A guide to using the de novo assembly tools in Geneious.
More Geneious Academy
TUTORIALPractice De Novo Assembly
Use this practical exercise to perform a de novo assembly of short-read NGS data and assemble circular contigs.
VIDEO SERIESMap to Reference
Use the map to reference tool to map next-generation sequencing data against a reference, and assemble coronavirus genomes.
VIDEO SERIESMultiple Alignments
Watch how to perform multiple alignments and assess levels of homology among sequences before constructing a phylogeny.
VIDEO SERIESSanger Sequence Analysis
Learn to align your chromatograms to a reference sequence, find variants and verify sequences in this video series.
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