Multiple Alignments in Geneious Prime

This video shows how to run a multiple sequence alignment in Geneious Prime, choosing from the built-in Geneious Aligner, Muscle, and Clustal Omega, or the MAFFT plugin, based on dataset size: Geneious Aligner for small datasets, Muscle for medium, and Clustal Omega or MAFFT for large alignments. Review the resulting alignment, including gaps from divergent or rapidly evolving sequences.

Introduction to Multiple Alignments

Run a multiple sequence alignment in Geneious Prime via Align/Assemble, choosing Geneious Aligner, Muscle, Clustal Omega, or the MAFFT plugin based on dataset size. Zoom in to expect large gaps where sequences are divergent or rapidly evolving.
LENGTH 2 minutes

Recommended Resources

What function should I use?

The difference between Pairwise/Multiple alignment, de novo Assembly, and Map to Reference.

Which multiple alignment algorithm should I use?

Four different multiple alignment algorithms are available in Geneious Prime.

Can I call SNPs on individual sequences aligned to a reference?

Learn how to call SNPs on the pairwise alignment/assembly.

Manual for Pairwise Alignment

A guide to using pairwise alignment in Geneious.

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