Sanger Sequence Analysis in Geneious Prime

Learn how to check sequence quality, trim the ends and map chromatogram sequences against a reference. Then view the contig and edit bases.

LENGTH 5 minutes

Set parameters for calling SNPs, view SNPs information, see how variants affect the protein, and view and export polymorphisms.

LENGTH 2 minutes

Recommended Resources

Use the Geneious de novo assembler to align pairs of Sanger sequences.

Identify the most appropriate assembly algorithm for your data with this decision tree.

This decision tree and validation table can help identify the most appropriate mapping algorithm for your data.

Learn how to view, edit, translate, complement and translate sequences.

Assemble bidirectional Sanger reads from blue tit mitochondrial DNA and extract high-quality consensus sequences.

Assemble short-read NGS data from scratch in Geneious Prime, including read normalization and circular contig assembly.

Fit a ladder, call peaks, bin alleles and produce a genotype table with the Microsatellite plugin.

Annotate your sequences by customizing the view, adding new annotations or annotating your sequence with BLAST.

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