Sanger Sequence Analysis

In this video series you will learn how to align your chromatograms to a reference sequence in Geneious Prime. Then use the Find Variations/SNPs tool to find variants and verify sequences.

Course Content

Introduction to Sanger Sequencing

INSIDE THE VIDEO
Learn how to check sequence quality, trim the ends and map chromatogram sequences against a reference. Then view the contig and edit bases.
LENGTH
5 minutes

Find Variants with Sanger Sequencing

INSIDE THE VIDEO
Set parameters for calling SNPs, view SNPs information, see how variants affect the protein, and view and export polymorphisms.
LENGTH
2 minutes

Recommended Resources

How do I assemble F and R pairs of sequences?

Use the Geneious de novo assembler to align pairs of Sanger sequences.

What Assembly Algorithm Ahould I Use?

Identify the most appropriate assembly algorithm for your data with this decision tree.

What Mapping Algorithm Should I Use?

This decision tree and validation table can help identify the most appropriate mapping algorithm for your data.

Manual for Creating, Viewing and Editing Sequences

Learn how to view, edit, translate, complement and translate sequences.

More Geneious Academy

TUTORIALAssemble Chromatograms and Edit Sequences in Geneious Prime
Learn how to assemble chromatograms, edit bidirectional DNA sequences, and extract high-quality consensus sequences.
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VIDEO SERIESDe Novo Assembly
Watch the series and learn how to perform a de novo assembly of short-read NGS data and assemble circular contigs.
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TUTORIALAnalyze Microsatellite Traces
Practice using the Microsatellite plugin to fit a ladder, call peaks, bin alleles and produce a table of genotypes.
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VIDEO SERIESAnnotate Sequences
Learn to annotate your sequences by customizing the view, adding new annotations or annotating your sequence with BLAST.
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