Expression Analysis

Calculate and compare normalized expression measures from RNA-Seq data using the Geneious expression analysis tool.

Course Content

Calculating Expression Levels

INSIDE THE VIDEO
Calculate normalized expression measures from RNA-Seq data using the Geneious expression analysis tool.
LENGTH
5 minutes

Measure Differential Expression

INSIDE THE VIDEO
Find differentially expressed genes, display confidence values, and filter results. View results in tabular format and volcano plots, or export the results as a CSV file.
LENGTH
6 minutes

Expression Analysis with DESeq2

INSIDE THE VIDEO
Use DESeq2 in Geneious Prime to compare expression levels for two sample conditions with replicates.
LENGTH
5 minutes

Recommended Resources

Can Geneious Prime handle RNA-Seq data?

How to map RNA-seq reads to a reference using the Geneious RNA assembler.

Setup Geneious Prime to analyse RNA-Seq using DESeq2, on Linux?

Analyze RNA-Seq data using DESeq2 within Geneious for Linux.

Manual for Analyzing Expression Levels

A guide to using the expression analysis tools in Geneious.

More Geneious Academy

Practice calculating normalized expression measures from RNA-Seq data using the Geneious expression analysis tool.
Use DESeq2 to compare expression levels for two sample conditions with replicates, in this practical exercise.
Use the map to reference tool to map next-generation sequencing data against a reference, and assemble coronavirus genomes.
In this video series learn to use workflows to automate your analyses and run a set of operations with a single click.
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