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Course Content
Calculating Expression Levels
Calculate normalized expression measures from RNA-Seq data using the Geneious expression analysis tool.
LENGTH 5 minutes
Measure Differential Expression
Find differentially expressed genes, display confidence values, and filter results. View results in tabular format and volcano plots, or export the results as a CSV file.
LENGTH 6 minutes
Expression Analysis with DESeq2
Use DESeq2 in Geneious Prime to compare expression levels for two sample conditions with replicates.
LENGTH 5 minutes
Recommended Resources
Can Geneious Prime handle RNA-Seq data?
How to map RNA-seq reads to a reference using the Geneious RNA assembler.
Setup Geneious Prime to analyse RNA-Seq using DESeq2, on Linux?
Analyze RNA-Seq data using DESeq2 within Geneious for Linux.
Manual for Analyzing Expression Levels
A guide to using the expression analysis tools in Geneious.
More Geneious Academy

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Measure RPKM, FPKM and TPM on RNA-Seq data from two sample conditions, then calculate differential expression.

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Use DESeq2 to find which Vibrio fischeri genes change expression during colonization of the bobtail squid.

VIDEO SERIESMap to Reference in Geneious Prime
Map next-generation sequencing data against a reference, and assemble coronavirus genomes.

VIDEO SERIESCreate Automated Workflows in Geneious Prime
Use workflows to automate your analyses and run a set of operations with a single click.
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