Find CRISPR Sites
- Locate CRISPR target sites and score them within a selected region of a genome or a stand alone gene sequence
- Batch discovery and scoring of CRISPR sites on sequence lists or groups of sequence documents
- Support for 3’ (Cas9) and 5’ (Cpf1) PAMs with customizable guide length and motif
- Automatically identify optimal pairs of CRISPR sites
- Assess sites quickly with innovative heatmap-style annotations based on your preferred scoring method
- Tabular view of candidates allows sorting and filtering of gRNAs and easy export for ordering or further analysis
- Evaluate on-target activity using the latest algorithms including Doench et al 2016
- Confidently pick the most specific guide RNA using scoring based on Zhang et al 2013
- Check for off-interactions in regions outside the selected gene and in a database of additional sequences
- Build a custom off-target database from your own specialized or proprietary data
Analyze CRISPR Editing Results
- Easily align, cluster and visualize NGS reads from your CRISPR editing experiments using a purpose-built algorithm
- Analyze the frequency of variants and their protein effects from your application of choice, including HDR, NHEJ and base editors
Finding CRISPR Sites – Learn how to find and annotate CRISPR sites in a sequence and check against a genome for off-target binding sites.
Analyzing CRISPR Editing – Learn how to analyze CRISPR editing results in Geneious Prime, including how to prepare your sequence files, set up the analysis and interpret the results.
More Geneious Prime Features
Molecular Cloning – View plasmid maps, automatically annotate vectors, find restriction sites; digest, ligate, and perform Golden Gate, Gibson, and Gateway cloning.
Primer Design – Design primers, find CRISPR sites, and optimize codons.
NGS Visualization and Analysis – Comprehensive analysis of data, including genome browser, contig visualization, SNP calling, and RNA-Seq expression analysis.
Alignment – Perform pairwise and multiple alignments of DNA or protein using trusted algorithms, including MAFFT and ClustalW.
Geneious has done a great deal for our lab, and I think it adds incredible value for labs with a mixture of wet and dry folks or those doing lots of cloning like ours.
Coming from the world of Vector NTI, I must tell you that Geneious looks and feel much better!
I find that Geneious is more than an invaluable tool for any modern molecular lab, it is an engine by itself that drives all our researches and speed up designs, strategies and innovations. And at my lab we are deeply interested not only to continue using this program but to always upgrade to the latest, and improved, releases