Microsatellite Analysis in Geneious Prime

Ensure your ladder is accurately called. Check the trim on your traces is set appropriately and that the correct peaks are called in the ladder.

LENGTH 4 minutes

Define the number of peaks, repeat units, and ranges for each locus. Add or remove additional loci and correct any incorrect or missing peaks.

LENGTH 2 minutes

Predict bins, resolve any unbinned peaks, view graphs, and export the resulting allele table as a CSV file.

LENGTH 2 minutes

Recommended Resources

Download the Microsatellite plugin for Geneious Prime.

Common questions on using microsatellite analysis in Geneious Prime.

Guide to using the microsatellite analysis tools in Geneious Prime.

Learn to use the Microsatellite Plugin to analyze traces from ABI fragment analysis machines.

Fit a ladder, call peaks, bin alleles and produce a genotype table with the Microsatellite plugin.

Align your chromatograms to a reference sequence, find variants and verify sequences.

Assemble bidirectional Sanger reads from blue tit mitochondrial DNA and extract high-quality consensus sequences.

Assemble short-read NGS data from scratch in Geneious Prime, including read normalization and circular contig assembly.

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