Microsatellite Analysis

Use the Microsatellites Plugin to import ABI fragment analysis files, visualize traces, fit ladders, call peaks and more.

INSIDE THE VIDEO

Ensure your ladder is accurately called. Check the trim on your traces is set appropriately and that the correct peaks are called in the ladder.

LENGTH

4 minutes

INSIDE THE VIDEO

Define the number of peaks, repeat units, and ranges for each locus. Add or remove additional loci and correct any incorrect or missing peaks.

LENGTH

2 minutes

INSIDE THE VIDEO

Predict bins, resolve any unbinned peaks, view graphs, and export the resulting allele table as a CSV file.

LENGTH

2 minutes

Recommended Resources

Download the Microsatellite plugin for Geneious Prime.

Common questions on using microsatellite analysis in Geneious Prime.

Guide to using the microsatellite analysis tools in Geneious Prime.

Learn to use the Microsatellite Plugin to analyze traces from ABI fragment analysis machines.

Practice using the Microsatellite plugin to fit a ladder, call peaks, bin alleles and produce a table of genotypes.

Learn to align your chromatograms to a reference sequence, find variants and verify sequences in this video series.

Practice how to trim, edit and assemble chromatograms. Find heterozygotes and incorrectly called bases.

Watch the series and learn how to perform a de novo assembly of short-read NGS data and assemble circular contigs.

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