Effortless NGS Visualization and Navigation
With just a click, get the visualization you need for the next generation sequencing data you have. Annotated genomes, circular genomes, mapped reads, contigs are all displayed in our highly customizable sequence view. Choose coloring schemes, graphs and tracks of additional annotations to display, then use dynamic annotation searching and location jumping to find what you’re looking for.
Powerful SNP Variants Analysis
Find SNP/Variants with either the versatile Geneious algorithm or well known FreeBayes. If you have variant calls from an external pipeline or provider, simply drag in a VCF. Identify novel variants by programmatic comparison with known variants or sort and filter using our dynamic tabular view then see the genomic context by switching to the synchronized sequence view.
Simple RNA-Seq Expression Analysis
Calculate expression levels from mapped reads then perform pairwise comparison between samples to identify differentially expressed genes. Without any expertise in the statistical programming language, R, use the industry standard DESeq2 package to compare expression between two conditions, each with replicate samples.
Switch to the PCA Plot tab to check the quality of your data using principal component analysis then switch to the interactive volcano plot to spot genes of interest. After selecting a gene in the volcano plot you can jump straight to it in the sequence view where all genes have heatmap coloring based on differential expression.
Expression Analysis – Learn to calculate normalized expression measures from RNA-Seq data. Measure RPKM, FPKM and TPM on datasets from two different sample conditions then calculate differential expression between the two samples.
Expression Analysis with DESeq2 – Learn how to use DESeq2 to compare expression levels for two sample conditions with replicates.
More Geneious Prime Features
Assembly and Mapping – De novo assembly or reference mapping using industry leading algorithms, including TopHat and Velvet.
Alignment – Perform pairwise and multiple alignments of DNA or protein using trusted algorithms, including MAFFT and ClustalW.
Analysis and Annotation – Trim, assemble, annotate and view Sanger sequencing trace files
Molecular Cloning – View plasmid maps, automatically annotate vectors, find restriction sites; digest, ligate, and perform Golden Gate, Gibson, and Gateway cloning.
BLAST / NCBI – Connect to NCBI and PubMed, submit sequences directly to GenBank, BLAST sequences and search your own database.
Import Data – Import and convert common file types as well as their annotations and notes with a simple drag and drop
I have found Geneious to be one of the most intuitive and powerful sequence analysis programs that I have used in all my years working in the molecular biology field. Thanks for making my life so much easier.
Geneious is an absolutely beautiful package to work with. We love it and we are telling everyone about it.
This program is wonderful! I was desperately looking for a software program that would make analyzing my sequencing data less painful. Thank you for developing a quality, user-friendly program. The interface is great!
Geneious is paramount in my research so I can’t be without it for too long.
The combination of powerful analytic features, intuitive navigation and file organization, and the responsiveness that representatives have shown to the inclusion of new functions has made us big fans of this software.
Geneious is a great software… I just want to express my appreciation to Biomatters to come up with such good product.
For many years I’ve wanted a friendly, menu-driven interface to our Glimmer program, but my research group has never had the resources available to build one. The Geneious Glimmer interface is terrific; it will allow any scientist to find genes in almost any bacterial, viral, or archaeal sequence, without requiring background expertise in Unix command-line programs. I’m also very pleased that Geneious makes Glimmer available across multiple computer platforms, including Windows and Mac.
We’ve been using our Geneious licenses a lot and really like the software. We’ve written several plug-ins ourselves and are using it more and more to analyze our data.
It’s not just about the buttons. From a user’s perspective, Phobos generates annotations, and Geneious handles annotations beautifully. The integration was obvious! And from a technical perspective it was easy to achieve, because it was simple to parse the outputs from Phobos into repeat annotations for the Geneious interface.
TopHat is a robust tool, but it was designed with experienced bioinformatics scientists in mind. Geneious makes TopHat accessible to many more users through the same powerful, intuitive user interface they have with other Geneious analysis tasks.