Sanger Sequence Analysis

Trim, assemble, and view Sanger sequencing trace files. Powerful SNP detection and variant calling.

View Chromatograms

View Sanger sequencing trace files. Navigate reads in a beautiful browser.

Assemble Chromatograms

Trim and align your Sanger sequencing traces. Improve your mapping accuracy and decrease your analysis time with simple sequence assembly and easy editing of contigs.

Powerful SNP detection and variant calling

Easily identify, examine and resolve disagreements manually in the sequence viewer.

  • Screen read errors by finding disagreements above a minimum threshold.
  • Find disagreements only in coding regions
  • Identify the effect of variations on protein translations, including those where only the first and third positions in a codon vary
  • Calculate the probability of a SNP being correct
  • Eliminate SNPs with a high strand bias
  • Export your variants to csv
  • Search and annotate short structural variations such short tandem repeats (STRs) and insertion and deletion events (InDels)




Learn: Assembly and Mapping

Learn how to assemble and map NGS reads and chromatogram sequences with videos and tutorials.

Learn: Sequence Analysis

Learn downstream analyses including expression analysis, metagenomics and microsatellite analysis.

Tutorial: Assembling Chromatograms

Learn how to edit and assemble chromatograms produced from a Sanger sequencing run


Sequence Annotation

Automatic genome annotation, real-time sequence analysis and protein structure prediction.

Primer Design

PCR and sequencing primer design tool. Primer database and testing.

Molecular Cloning

View plasmid maps, annotate vectors, find restriction sites. Simulate restriction, Golden Gate, Gibson, and Gateway cloning.

Import Data

Import and convert common file types as well as their annotations and notes with a simple drag and drop

I have found Geneious to be one of the most intuitive and powerful sequence analysis programs that I have used in all my years working in the molecular biology field. Thanks for making my life so much easier.
Prof. John PayneUniversity of California