Geneious Prime
Geneious Biologics
Gateway Cloning
Learn how Gateway cloning works and how to clone single or multiple fragments into an expression clone.
Performing Gateway Cloning with a Single Insert
INSIDE THE VIDEO
Perform BP and LR reactions, design primers to amplify your sequence, incorporate AttB sites, and simulate a one-step Gateway reaction.
- How gateway cloning works
- Steps 1- 3 of the gateway cloning method
- One-step gateway reaction
LENGTH
9 minutes
Gateway Cloning with Multiple Inserts
INSIDE THE VIDEO
Understand the concepts behind multisite Gateway cloning, choose attB sites for correct insertion, design primers, and simulate BP and LR reactions.
- How multisite gateway cloning works
- Steps 1 – 3 in the multisite gateway cloning method
LENGTH
7 minutes
Recommended Resources
GATEWAY CLONING
TUTORIAL
Practice Gateway Cloning
Learn to perform Gateway Cloning with this series of practical exercises.
FAQ
How to add primers to Geneious Prime?
Add existing primers to Geneious Prime, either manually or by importing from external sources.
VIDEO SERIES
How To Design Primers
Learn how to view, design and export primers for PCR, cloning or sequencing.
GENEIOUS ACADEMY
